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在运动饮料中添加纳米级羟基磷灰石以抑制牙齿侵蚀:使用牛牙釉质的体外研究。

The addition of nano-sized hydroxyapatite to a sports drink to inhibit dental erosion: in vitro study using bovine enamel.

机构信息

Department of Preventive Dentistry and Public Oral Health, Research Center for Orofacial Hard Tissue Regeneration, College of Dentistry, Yonsei University, Seoul, Republic of Korea.

出版信息

J Dent. 2011 Sep;39(9):629-35. doi: 10.1016/j.jdent.2011.07.001. Epub 2011 Jul 7.

Abstract

OBJECTIVES

This study examined the dental erosion and demineralization potential of a sports drink containing nano-sized hydroxyapatite (nano-HA) as an additive.

METHODS

The experimental solutions were Powerade (PA) alone and PA with 0.05%, 0.10%, and 0.25% nano-HA. The pH, titratable acidity, and calcium and phosphate content of each solution were analysed, and the degree of saturation with respect to the dental enamel (DS(En)) was obtained. Twelve sound bovine enamel specimens for each group were treated in accordance with the pH-cycling schedule which had 60min treatment with experimental solution per day for 7 days. The erosion potential was determined from the changes in surface micro hardness (SMH), the depths of erosion and demineralized layer using confocal laser scanning microscopy (CLSM), and the morphological changes to the tooth surface were examined with scanning electron microscopy (SEM) after pH-cycling.

RESULTS

pH and DS(En) increased with increasing nano-HA concentration in the drinks, whereas the titratable acidity decreased. There were significant differences in the SMH between the PA alone and >0.10% nano-HA groups (p<0.001). Although the PA alone group showed a pronounced erosion depth, CLSM showed no erosion depth in 0.25% nano-HA group. SEM showed an intact surface with increasing nano-HA concentration in the drinks. In conclusion, dental erosion was effectively prevented with increase of adding concentration of nano-HA, and a sports drink containing 0.25% nano-HA might prevent dental erosion.

摘要

目的

本研究考察了含有纳米羟基磷灰石(nano-HA)作为添加剂的运动饮料的牙齿侵蚀和脱矿潜力。

方法

实验溶液为单独的 Powerade(PA)以及含有 0.05%、0.10%和 0.25%纳米-HA 的 PA。分析了每种溶液的 pH 值、可滴定酸度以及钙和磷酸盐含量,并获得了相对于牙釉质的饱和度(DS(En))。每组 12 个健康牛牙釉质标本按照 pH 循环方案进行处理,每天用实验溶液处理 60 分钟,共 7 天。通过表面显微硬度(SMH)的变化、侵蚀和脱矿层的深度来确定侵蚀潜力,使用共聚焦激光扫描显微镜(CLSM)进行测量,并用扫描电子显微镜(SEM)检查牙齿表面的形态变化。

结果

pH 值和 DS(En)随饮料中纳米-HA 浓度的增加而增加,而可滴定酸度则降低。在单独的 PA 和 >0.10%纳米-HA 组之间,SMH 存在显著差异(p<0.001)。尽管单独的 PA 组显示出明显的侵蚀深度,但 CLSM 显示在 0.25%纳米-HA 组中没有侵蚀深度。SEM 显示随着饮料中纳米-HA 浓度的增加,表面完整。总之,随着纳米-HA 添加浓度的增加,牙齿侵蚀得到了有效预防,含有 0.25%纳米-HA 的运动饮料可能预防牙齿侵蚀。

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