Department of PG Studies and Research in Industrial Chemistry, Kuvempu University, Shimoga, Karnataka, India.
Colloids Surf B Biointerfaces. 2011 Nov 1;88(1):413-8. doi: 10.1016/j.colsurfb.2011.07.023. Epub 2011 Jul 18.
L-arginine was electropolymerised on a carbon paste electrode (CPE) to form the biopolymer by free radical formation in the electro oxidation process of the amino and carboxylic group containing compound by cyclic voltammetric technique. The modified electrode shows an excellent electrocatalytic activity towards the oxidation of both dopamine (DA) and ascorbic acid (AA). It was demonstrated that the deposited biopolymer has positive charges over the bare carbon electrode surface, which leads to the formation of electrical double layer made the fast electron transfer process could leads to the diffusion of dopamine, ascorbic acid and uric acid on their charge gradient by cyclic voltammetric technique. The response of the sensor was tested towards the different dopamine concentration. The catalytic peak current obtained was linearly related to DA concentrations in the ranges of 5×10(-5) to 1×10(-4)M L(-1) with correlation co-efficient of 0.9924 which reveals the adsorption controlled process. The detection limit for dopamine was 5×10(-7)M L(-1). The interference studies showed that the modified electrode exhibits excellent selectivity in the presence of large excess of ascorbic acid (AA) and response is fast stable, reliable, resistant to biofouling and can be applied for the real sample analysis in medical, pharmaceutical and biotechnological sectors. The adsorption-controlled process and kinetic parameters of the poly(L-arginine) were determined using electrochemical approaches.
L-精氨酸在碳糊电极（CPE）上通过自由基形成进行电聚合，从而在含有氨基和羧基的化合物的电氧化过程中形成生物聚合物。修饰后的电极对多巴胺（DA）和抗坏血酸（AA）的氧化表现出优异的电催化活性。证明沉积的生物聚合物在裸碳电极表面带有正电荷，这导致形成双电层，使快速电子转移过程能够导致多巴胺、抗坏血酸和尿酸在其电荷梯度上扩散。通过循环伏安技术测试了传感器对不同多巴胺浓度的响应。获得的催化峰电流与 DA 浓度在 5×10(-5)到 1×10(-4)M L(-1)的范围内呈线性关系，相关系数为 0.9924，这表明是吸附控制过程。多巴胺的检测限为 5×10(-7)M L(-1)。干扰研究表明，在存在大量抗坏血酸（AA）的情况下，修饰后的电极表现出优异的选择性，并且响应快速稳定、可靠、抗生物污染，可用于医学、制药和生物技术领域的实际样品分析。使用电化学方法确定了聚（L-精氨酸）的吸附控制过程和动力学参数。
