Department of Radiology, University of Minnesota, Minneapolis, MN 55455, USA.
J Vasc Interv Radiol. 2011 Oct;22(10):1464-1470.e2. doi: 10.1016/j.jvir.2011.06.010. Epub 2011 Aug 4.
To develop and evaluate a bioresorbable spherical material for embolization.
New bioresorbable hydrogel microspheres were prepared from carboxymethyl cellulose and chitosan by using an inverse emulsion method. Size distribution of the microspheres was determined with a microscope, and the colorability was tested with Evans blue dye. The compressibility was examined with a texture analyzer. After sieving, the suspendability of the microspheres was tested in saline solution/contrast agent mixture in different ratios, and then the injectability was tested with microcatheters (lumen sizes of 0.0165, 0.019, and 0.027 inches). The in vitro degradability tests were performed in a lysozyme solution. Cell culture study of the microspheres was performed with human fibroblasts.
The microspheres exhibit diameters of 100-1,550 μm with a transparent appearance. Their fracture force can reach 0.58-0.88 N, and fracture deformation varies from approximately 70% to 95% of their original size. These microspheres can be colored by Evans blue dye, and uniform subgroups of microspheres can be readily obtained by sieving. Within 3 minutes, the microspheres form a stable suspension in a 4:6 contrast agent/saline solution mixture, which can be easily injected through microcatheters without aggregating or clogging. These microspheres are biodegradable, with degradation times varying from 2 weeks to 1 month, depending on their composition. Cell culture studies reveal no adverse effect on the growth of human fibroblasts in the presence of the microspheres.
A biodegradable and noncytotoxic microsphere was successfully prepared. It can be well suspended in the contrast solution and easily injected through a microcatheter.
开发并评估一种可生物吸收的球形栓塞材料。
采用反相乳液法，由羧甲基纤维素和壳聚糖制备新型可生物吸收的水凝胶微球。使用显微镜测定微球的粒径分布，用 Evans 蓝染料测试其着色性。用质构仪测定压缩性。筛分后，在不同比例的盐溶液/对比剂混合物中测试微球的悬浮性，然后用微导管（内径分别为 0.0165、0.019 和 0.027 英寸）测试可注射性。在溶菌酶溶液中进行体外降解试验。用人成纤维细胞进行微球的细胞培养研究。
微球呈透明外观，粒径为 100-1550μm。其断裂力可达 0.58-0.88N，断裂变形约为原始尺寸的 70%-95%。这些微球可以用 Evans 蓝染料着色，通过筛分可以很容易地获得均匀的微球亚群。在 3 分钟内，微球在 4:6 的对比剂/盐溶液混合物中形成稳定的悬浮液，可轻松通过微导管注入，不会聚集或堵塞。这些微球具有可降解性，降解时间取决于其组成，从 2 周到 1 个月不等。细胞培养研究表明，微球存在时对人成纤维细胞的生长没有不良影响。
成功制备了一种可生物降解且无细胞毒性的微球。它可以很好地悬浮在对比溶液中，并通过微导管轻松注射。
