College of Marine Life Science, Ocean University of China, Qingdao 266003, China; Key Laboratory for Nano-Bio Interface Research, Suzhou Key Laboratory for Nanotheranostics, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
College of Marine Life Science, Ocean University of China, Qingdao 266003, China.
Carbohydr Polym. 2014 Nov 26;113:304-13. doi: 10.1016/j.carbpol.2014.06.080. Epub 2014 Jul 11.
To evaluate the potential of N-acetylated chitosan microspheres used as a chemoembolic agent in vivo and in vitro. Calibrated spherical chitosan microspheres (CMs) were prepared via Water-in-Oil emulsification method and CMs were acetylated (ACMs). The swelling rate of CMs was greatly affected by pH than that of ACMs and both of them affected by temperature. Microspheres with excellent thermal stability demonstrated controllable degradation in lysozyme solution. Doxorubicin was released from microspheres in vitro and exhibited excellent control release profile. ACMs caused hemolysis less than CMs (<5% of the time). Co-culture with mouse embryo fibroblasts revealed that microspheres have non-cytotoxic nature. Microspheres planted in a rat gluteal muscle demonstrated that it were biodegradable and biocompatible. ACMs were performed in rabbit ear embolization model and ischemic necrosis on ear was visible due to the vascular occlusion after 15 days. Acetylated chitosan microspheres could be used as potential biocompatible and biodegradable embolic agents.
评估 N-乙酰化壳聚糖微球作为体内和体外化学栓塞剂的潜力。通过水包油乳化法制备了校准的球形壳聚糖微球（CMs），并对其进行了乙酰化（ACMs）。CMs 的溶胀率受 pH 值的影响大于 ACMs，两者均受温度的影响。具有优异热稳定性的微球在溶菌酶溶液中表现出可控制的降解。阿霉素从微球中外泄，表现出优异的控制释放特性。ACMs 引起的溶血少于 CMs（时间不到 5%）。与小鼠胚胎成纤维细胞共培养表明微球具有非细胞毒性。微球种植在大鼠臀肌中表明其具有生物可降解性和生物相容性。ACMs 在兔耳栓塞模型中进行，15 天后由于血管阻塞，可观察到耳缺血性坏死。N-乙酰化壳聚糖微球可用作有潜力的生物相容性和生物可降解栓塞剂。
