一种普遍适用的用于在动物体内追踪碳纳米管的放射性示踪方法。
A generally adoptable radiotracing method for tracking carbon nanotubes in animals.
作者信息
Deng Xiaoyong, Yang Shengtao, Nie Haiyu, Wang Haifang, Liu Yuanfang
机构信息
Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, People's Republic of China. Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai 200444, People's Republic of China.
出版信息
Nanotechnology. 2008 Feb 20;19(7):075101. doi: 10.1088/0957-4484/19/7/075101. Epub 2008 Jan 29.
Carbon nanotube (CNT) mediated drug delivery systems have currently aroused a great deal of interest. Such delivery systems for drugs, proteins and genes have been preliminarily studied using cellular and animal models. For the further study of the pharmacokinetics and related biological behaviours of CNTs in vivo, a fast and convenient tracing method is particularly demanded. In this paper, we developed a generally adoptable tracing method for the biodistribution study of functionalized CNTs in vivo. Taurine covalently functionalized multi-walled carbon nanotubes (tau-MWNTs) and Tween-80 wrapped MWNTs (Tween-MWNTs) were labelled with (125)I, and then their distribution in mice was determined. It is interesting that Tween-80 can reduce the RES uptake of MWNTs remarkably. The resulting distribution of (125)I-tau-MWNTs was very consistent with that using (14)C-taurine-MWNTs as the CNTs tracer, which means the easy (125)I labelling method is reliable and effective.
碳纳米管（CNT）介导的药物递送系统目前已引起了广泛关注。已使用细胞和动物模型对这类药物、蛋白质和基因递送系统进行了初步研究。为了进一步研究CNT在体内的药代动力学及相关生物学行为，特别需要一种快速便捷的追踪方法。在本文中，我们开发了一种普遍适用的追踪方法，用于体内功能化CNT的生物分布研究。用¹²⁵I标记牛磺酸共价功能化的多壁碳纳米管（tau-MWNTs）和吐温80包裹的MWNTs（吐温-MWNTs），然后测定它们在小鼠体内的分布。有趣的是，吐温80可显著降低MWNTs对网状内皮系统（RES）的摄取。¹²⁵I-tau-MWNTs的最终分布与使用¹⁴C-牛磺酸-MWNTs作为CNT示踪剂的分布非常一致，这意味着简便的¹²⁵I标记方法可靠且有效。
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