Strojny Barbara, Kurantowicz Natalia, Sawosz Ewa, Grodzik Marta, Jaworski Sławomir, Kutwin Marta, Wierzbicki Mateusz, Hotowy Anna, Lipińska Ludwika, Chwalibog André
Division of Nanobiotechnology, Warsaw University of Life Sciences, Warsaw, Poland.
Department of Chemical Technologies, Institute of Electronic Materials Technology, Warsaw, Poland.
PLoS One. 2015 Dec 14;10(12):e0144821. doi: 10.1371/journal.pone.0144821. eCollection 2015.
Due to their excellent biocompatibility, carbon nanoparticles have been widely investigated for prospective biomedical applications. However, their impact on an organism with prolonged exposure is still not well understood. Here, we performed an experiment investigating diamond, graphene oxide and graphite nanoparticles, which were repeatedly administrated intraperitoneally into Wistar rats for four weeks. Some of the animals was sacrificed after the last injection, whereas the rest were sacrificed twelve weeks after the last exposure. We evaluated blood morphology and biochemistry, as well as the redox and inflammatory state of the liver. The results show the retention of nanoparticles within the peritoneal cavity in the form of prominent aggregates in proximity to the injection site, as well as the presence of some nanoparticles in the mesentery. Small aggregates were also visible in the liver serosa, suggesting possible transportation to the liver. However, none of the tested nanoparticles affected the health of animals. This lack of toxic effect may suggest the potential applicability of nanoparticles as drug carriers for local therapies, ensuring accumulation and slow release of drugs into a targeted tissue without harmful systemic side effects.
由于其优异的生物相容性，碳纳米颗粒已被广泛研究用于潜在的生物医学应用。然而，它们长期暴露对生物体的影响仍未得到充分了解。在此，我们进行了一项实验，研究了金刚石、氧化石墨烯和石墨纳米颗粒，将它们反复腹腔注射到Wistar大鼠体内，持续四周。在最后一次注射后，处死了一些动物，而其余动物在最后一次暴露后十二周被处死。我们评估了血液形态和生化指标，以及肝脏的氧化还原和炎症状态。结果显示，纳米颗粒以明显聚集体的形式保留在腹腔内，靠近注射部位，并且在肠系膜中也存在一些纳米颗粒。在肝脏浆膜中也可见小聚集体，表明可能转运至肝脏。然而，所测试的纳米颗粒均未影响动物的健康。这种缺乏毒性作用可能表明纳米颗粒作为局部治疗药物载体的潜在适用性，可确保药物在靶向组织中积累并缓慢释放，而无有害的全身副作用。
