Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Chem Commun (Camb). 2011 Sep 28;47(36):10055-7. doi: 10.1039/c1cc13367e. Epub 2011 Aug 5.
We studied the photobleaching of a library of boron dipyrromethene (BODIPY) derivatives with a range of electron densities, and found that the photobleaching rate is influenced by the electron-withdrawing capacity of the substituents. Electron-deficient BODIPYs generated less singlet oxygen, were less reactive to singlet oxygen, and were highly resistant to photobleaching. We confirmed the utility of one of these fluorophores, 2,6-diCO(2)R-BDP, for visualizing EGF receptor dynamics in cells expressing an SNAP-tagged EGF receptor.
我们研究了一系列电子密度不同的硼二吡咯亚甲基（BODIPY）衍生物的光漂白，发现光漂白速率受取代基的吸电子能力影响。缺电子的 BODIPY 产生的单线态氧较少，对单线态氧的反应性较低，且对光漂白具有高度抗性。我们证实了其中一种荧光染料 2,6-二 CO(2)R-BDP 的实用性，可用于可视化表达 SNAP 标签 EGF 受体的细胞中 EGF 受体的动力学。
