Centre for Bioactive Chemistry, Department of Chemistry, University of Durham, Science Laboratories, South Road, Durham, DH1 3LE, UK.
Plant J. 2010 May;62(3):529-38. doi: 10.1111/j.1365-313X.2010.04153.x. Epub 2010 Jan 25.
There is a continuing need for bioprobes that are target-specific and combine speed of delivery with maintenance of normal cell behaviour. Towards this end, we are developing small pro-fluorescent molecules that provide such specificity through chemical activation by biomolecules. We have generated a set of BODIPY (boron dipyrromethane) fluorophores, including one that is intrinsically non-fluorescent but on incubation with cells becomes fluorescent at its target site. Addition of these BODIPY probes to plant cells identifies peroxisomes, as verified by co-localization with an SKL-FP construct. Interestingly, in mammalian cells, co-localization with the mammalian peroxisomal marker SelectFX(TM) was not observed. These data suggest fundamental differences in peroxisome composition, development or function between plant and animal cells.
目前仍需要能够特异性靶向特定目标，并且兼具快速递达和维持正常细胞行为的生物探针。为此，我们正在开发一些小的荧光前体分子，通过生物分子的化学激活来提供这种特异性。我们已经生成了一组 BODIPY（硼二吡咯甲烷）荧光团，其中包括一个本来没有荧光的，但在与细胞孵育时在其靶位变得有荧光的分子。将这些 BODIPY 探针添加到植物细胞中，可以通过与 SKL-FP 构建体的共定位来鉴定过氧化物酶体。有趣的是，在哺乳动物细胞中，没有观察到与哺乳动物过氧化物酶体标记物 SelectFX（TM）的共定位。这些数据表明，植物细胞和动物细胞的过氧化物酶体在组成、发育或功能上存在根本差异。
