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纹状体中的多巴胺含量和行为异常老年大鼠 Bad 和 Bcl-2 的表达变化。

Dopamine content in the striatum and expression changes of Bad and Bcl-2 in elderly rats with abnormal behavior.

机构信息

Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.

出版信息

Neurochem Res. 2011 Dec;36(12):2333-8. doi: 10.1007/s11064-011-0558-3. Epub 2011 Aug 5.

DOI:10.1007/s11064-011-0558-3
PMID:21818658
Abstract

To determine the dopamine (DA) content in the striatum and the expression changes of the apoptosis-associated proteins Bad and Bcl-2 in the substantia nigra compacta (SNc) in elderly rats with abnormal behavior. Fifty three Sprague-Dawley rats were divided into three groups: adult, age-motorplus (normal behavior) and aged-motorminus (abnormal behavior) using the hanger test. The DA content in the striatum and the expression of tyrosine hydroxylase (TH), Bad and Bcl-2 in the SNc were measured by HPLC/MS (high performance liquid chromatogram-mass spectra) and Western Blot. (1) The results from the hanger test demonstrated that the scores and latency of aged-motorminus group were lower than the age-motorplus group. (2) Results from HPLC-MS showed that, compared with the age-motorplus and adult group, the content of DA in elderly rat striata decreased significantly, with a statistically significant difference. (3) The Western Blot demonstrated that, compared with the adults, the expression of TH in elderly rats significantly decreased, but the difference was not significant between the aged-motorminus group and the age-motorplus group. Compared with the age-motorplus and the adult group, the expression of Bad increased but Bcl-2 decreased in the aged-motorminus group. The decrease in TH content in the SNc correlated with the aging of rats. The decrease in DA content in the striatum may correlate with the abnormal behavior in elderly rats, which could be ascribed to the variations in Bad and Bcl-2.

摘要

目的

探讨行为异常老年大鼠纹状体多巴胺（DA）含量及黑质致密部（SNc）中凋亡相关蛋白 Bad、Bcl-2 表达变化。

方法

采用悬挂实验将 53 只 SD 大鼠分为成年组、同龄运动正常组和同龄运动异常组。采用高效液相色谱-质谱（HPLC-MS）和 Western blot 法检测各组大鼠纹状体 DA 含量及 SNc 中酪氨酸羟化酶（TH）、Bad、Bcl-2 表达。

结果

（1）悬挂实验结果：同龄运动异常组大鼠的得分和潜伏期均低于同龄运动正常组。（2）HPLC-MS 结果：与同龄运动正常组和成年组比较，老年大鼠纹状体 DA 含量明显降低，差异有统计学意义。（3）Western blot 结果：与成年组比较，老年大鼠 SNc 中 TH 表达明显降低，但同龄运动异常组与同龄运动正常组比较差异无统计学意义。与同龄运动正常组和成年组比较，同龄运动异常组 Bad 表达增加，Bcl-2 表达降低。

结论

SNc 中 TH 含量的降低可能与大鼠的衰老有关，纹状体 DA 含量的降低可能与老年大鼠的异常行为有关，其机制可能与 Bad、Bcl-2 的变化有关。

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2
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3
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