[缩泉胶囊对肾虚利尿大鼠CYP11B2 mRNA表达的影响]
[The influence of suoquan capsule on the mRNA expression of CYP11B2 in deficiency of the kidney and diuresis rats].
作者信息
Li Shu-wen, Hu Lv-jiang, Wu Qing-he, Huang Ping, Cao Hong-ying
机构信息
Jiangxi College of Traditional Chinese Medicine, Fuzhou 344000, China.
出版信息
Zhong Yao Cai. 2011 Jan;34(1):80-3.
OBJECTIVE
To explore the mechanism of nourishing kidney and reducing urine effect of suoquan capsule by discussing the adjusting action on ALD synthase.
METHODS
Built the model of deficiency of the kidney and diuresis by adenine,inspectd the contents of Cort, ALD in blood and the mRNA expression of CYP11B2 with ELISA and RT-PCR technology of the mice.
RESULTS
Compared with model group, Suoquan capsule could remarkedly increase the contents of Cort, ALD in blood and the mRNA expression of CYP1 1 B2 of model rats.
CONCLUSIONS
Suoquan capsule can increase the contents of Cort, ALD in blood and the mRNA expression of CYP11B2 in deficiency of the kidney and diuresis rats. Promote the combining of ALD by adjusting the ALD synthase may be the mechanism of nourishing the kidney and reducing urine of Souquan capsule from ALD synthase approach.
目的
通过探讨缩泉胶囊对醛固酮合酶（ALD synthase）的调节作用，研究其补肾缩尿的作用机制。
方法
采用腺嘌呤建立肾虚多尿模型，运用ELISA和RT-PCR技术检测小鼠血液中皮质醇（Cort）、醛固酮（ALD）含量及CYP11B2 mRNA表达。
结果
与模型组比较，缩泉胶囊可显著提高模型大鼠血液中Cort、ALD含量及CYP11B2 mRNA表达。
结论
缩泉胶囊可提高肾虚多尿大鼠血液中Cort、ALD含量及CYP11B2 mRNA表达。从醛固酮合酶途径调节醛固酮合酶，促进醛固酮结合，可能是缩泉胶囊补肾缩尿的作用机制。
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