• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

使用单一特异性引物的聚合酶链反应(PCR)扩增。

Polymerase chain reaction (PCR) amplification with a single specific primer.

作者信息

Kalman M, Kalman E T, Cashel M

机构信息

Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.

出版信息

Biochem Biophys Res Commun. 1990 Mar 16;167(2):504-6. doi: 10.1016/0006-291x(90)92052-2.

DOI:10.1016/0006-291x(90)92052-2
PMID:2182014
Abstract

A method is described for amplification of DNA fragments flanking a single known sequence that is sufficiently long to enable synthesis of a functional primer in polymerase chain reactions.

摘要

本文描述了一种用于扩增单个已知序列侧翼DNA片段的方法,该已知序列足够长,能够在聚合酶链反应中合成功能性引物。

相似文献

1
Polymerase chain reaction (PCR) amplification with a single specific primer.使用单一特异性引物的聚合酶链反应(PCR)扩增。
Biochem Biophys Res Commun. 1990 Mar 16;167(2):504-6. doi: 10.1016/0006-291x(90)92052-2.
2
Characterization of contaminating DNA in Taq polymerase which occurs during amplification with a primer set for Legionella 5S ribosomal RNA.用嗜肺军团菌5S核糖体RNA引物组扩增过程中Taq聚合酶中污染DNA的特性分析
Mol Cell Probes. 1994 Feb;8(1):11-4. doi: 10.1006/mcpr.1994.1002.
3
Rapid polymerase chain reaction amplification using intact bacterial cells.使用完整细菌细胞进行快速聚合酶链反应扩增。
Biotechniques. 1991 Jan;10(1):42, 44-5.
4
Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids.通过反向PCR从环境DNA中检索完整基因,并使用含有锁核酸的引物对目标基因进行预扩增。
Environ Microbiol. 2008 Apr;10(4):978-87. doi: 10.1111/j.1462-2920.2007.01518.x. Epub 2007 Dec 17.
5
A simple DNA polymerase chain reaction method to locate and define orientation of specific sequences in cloned bacterial genomic fragments.一种用于定位和确定克隆的细菌基因组片段中特定序列方向的简单DNA聚合酶链反应方法。
Microbios. 1993;75(303):125-9.
6
Amplification and sequencing of end fragments from bacterial artificial chromosome clones by single-primer polymerase chain reaction.
Anal Biochem. 1997 Nov 15;253(2):272-5. doi: 10.1006/abio.1997.2404.
7
Rapid genome walking: a simplified oligo-cassette mediated polymerase chain reaction using a single genome-specific primer.快速基因组步移:一种使用单一基因组特异性引物的简化寡核苷酸盒介导的聚合酶链反应。
Nucleic Acids Res. 2000 Jun 1;28(11):E55. doi: 10.1093/nar/28.11.e55.
8
DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting.通过任意引物PCR(随机扩增多态性DNA)指纹图谱检测大肠杆菌K-12衍生物之间的DNA序列差异。
J Bacteriol. 1994 Mar;176(6):1673-82. doi: 10.1128/jb.176.6.1673-1682.1994.
9
DNA microarrays for comparative genomic hybridization based on DOP-PCR amplification of BAC and PAC clones.基于BAC和PAC克隆的DOP-PCR扩增用于比较基因组杂交的DNA微阵列。
Genes Chromosomes Cancer. 2003 Apr;36(4):361-74. doi: 10.1002/gcc.10155.
10
Efficiency of the polymerase chain reaction amplification of the uid gene for detection of Escherichia coli in contaminated water.用于检测污水中大肠杆菌的uid基因聚合酶链反应扩增效率
Lett Appl Microbiol. 1997 Jun;24(6):498-502. doi: 10.1046/j.1472-765x.1997.00160.x.

引用本文的文献

1
Supported PCR: an efficient procedure to amplify sequences flanking a known DNA segment.支持性PCR:一种扩增已知DNA片段侧翼序列的有效方法。
Plant Mol Biol. 1993 Feb;21(4):723-8. doi: 10.1007/BF00014557.
2
Isolation and detection of urease genes in Ureaplasma urealyticum.解脲脲原体中脲酶基因的分离与检测
Infect Immun. 1991 Jul;59(7):2463-9. doi: 10.1128/iai.59.7.2463-2469.1991.
3
PCR walking from microdissection clone M54 identifies three exons from the human gene for the neural cell adhesion molecule L1 (CAM-L1).从显微切割克隆M54进行的聚合酶链反应步移鉴定出了人类神经细胞黏附分子L1(CAM-L1)基因的三个外显子。
Nucleic Acids Res. 1991 Oct 11;19(19):5395-401. doi: 10.1093/nar/19.19.5395.
4
In situ amplification of single copy gene segments in individual cells by the polymerase chain reaction.
Infection. 1991 Jul-Aug;19(4):242-4. doi: 10.1007/BF01644953.
5
The polymerase chain reaction and other amplification techniques in immunological research and diagnosis.聚合酶链反应及免疫研究与诊断中的其他扩增技术。
Immunology. 1992 Jan;75(1):3-9.
6
Genomic structure of the human caldesmon gene.人类钙调蛋白基因的基因组结构。
Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):12122-6. doi: 10.1073/pnas.89.24.12122.