使用单一特异性引物的聚合酶链反应（PCR）扩增。
Polymerase chain reaction (PCR) amplification with a single specific primer.
作者信息
Kalman M, Kalman E T, Cashel M
机构信息
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
出版信息
Biochem Biophys Res Commun. 1990 Mar 16;167(2):504-6. doi: 10.1016/0006-291x(90)92052-2.
PMID:2182014
Abstract
A method is described for amplification of DNA fragments flanking a single known sequence that is sufficiently long to enable synthesis of a functional primer in polymerase chain reactions.
摘要
本文描述了一种用于扩增单个已知序列侧翼DNA片段的方法，该已知序列足够长，能够在聚合酶链反应中合成功能性引物。
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