Psychiatric Centre Copenhagen, Rigshospitalet O-6102, Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
Neuropeptides. 2011 Oct;45(5):337-41. doi: 10.1016/j.npep.2011.07.004. Epub 2011 Aug 4.
Induction of seizures by electroconvulsive stimulation (ECS) is amongst the most efficacious treatments for major depression. However, the working mechanism by which ECS exerts its antidepressant effects remains elusive. The galanin system is regulated by ECS in seizure-prone brain regions and has been shown to modulate depression-like behaviour. To further explore its potential role in the antidepressant effects of ECS the galanin system was investigated by in situ hybridisation and [(125)I]-galanin receptor binding during repeated ECS in the locus coeruleus, dorsal raphe and discrete nuclei of the hypothalamus. Adult mice were treated with ECS once daily for 14 consecutive days, a paradigm previously shown to exert antidepressant-like effects. Significant increases in galanin transcription were found in the locus coeruleus and dorsomedial nuclei of the hypothalamus. In addition, GalR2 mRNA levels in the ventro- and dorsomedial nuclei of the hypothalamus were upregulated whereas no GalR1 mRNA upregulation was observed. [(125)I]-galanin receptor binding was downregulated in the ventromedial nuclei of the hypothalamus and dorsal raphe. These data show that the galanin system is regulated by repeated ECS in brain regions involved in monoaminergic neurotransmission and stress modulation thus indicating a possible role of the galanin system in the therapeutic effects of ECS.
电惊厥刺激 (ECS) 诱发癫痫发作是治疗重度抑郁症最有效的方法之一。然而，ECS 发挥抗抑郁作用的工作机制仍然难以捉摸。甘丙肽系统受 ECS 在易发生癫痫的脑区的调节，并且已经显示出调节抑郁样行为的作用。为了进一步探索其在 ECS 抗抑郁作用中的潜在作用，通过原位杂交和 [(125)I]-甘丙肽受体结合，在蓝斑核、中缝背核和下丘脑离散核中重复 ECS 期间研究了甘丙肽系统。成年小鼠每天接受 ECS 治疗一次，连续 14 天，这一方案先前已显示出抗抑郁样作用。在蓝斑核和下丘脑背内侧核中发现甘丙肽转录显著增加。此外，下丘脑腹侧和背侧内侧核中的 GalR2 mRNA 水平上调，而 GalR1 mRNA 没有上调。[(125)I]-甘丙肽受体结合在下丘脑腹内侧核和中缝背核下调。这些数据表明，甘丙肽系统受重复 ECS 在涉及单胺能神经传递和应激调节的脑区调节，因此表明甘丙肽系统在 ECS 的治疗效果中可能起作用。
