Institut Lavoisier, UMR 8180 CNRS-Université de Versailles Saint Quentin en Yvelines, 45 avenue des Etats Unis, 78035 Versailles Cedex, France.
J Pharm Biomed Anal. 2011 Dec 5;56(4):758-62. doi: 10.1016/j.jpba.2011.07.011. Epub 2011 Jul 21.
Quantification of fumaric acid, an endogenous dicarboxylic acid with interesting biomedical applications either through its own biological activity or as a linker constitutive of the porous iron(III) fumarate metal organic framework (MOF) MIL-88A based drug nanocarrier (MIL stands for Material from Institut Lavoisier), has been developed in different rat biological complex media (liver, spleen and urine). After a liquid-liquid extraction procedure, fumaric acid concentration was determined by a simple and accurate high-performance liquid chromatography (HPLC) method coupled to a photodiode-array detector (PDA) using aminosalicylic acid as internal standard (IS) and a gradient elution. The recovery of fumaric acid reaches 89% and 92% for urine (for concentrations of 0.05 and 1μgml(-1), respectively) and 90% for liver and spleen tissues, exceeding 89% in all instances in comparison with the IS. Linearity has been kept from 0.05 to 1μgml(-1) and from 0.5 to 10μgg(-1) of fumaric acid in urine and tissues, respectively. The limit of detection of the method was 0.01μg per injection. This method has finally allowed the quantification of fumaric acid in rat urine and tissue samples after the intravenous administration of MIL-88A nanoparticles.
已在不同的大鼠生物复杂介质（肝、脾和尿）中开发了富马酸（一种具有有趣的生物医学应用的内源性二羧酸，无论是通过其自身的生物活性还是作为多孔铁（III）富马酸盐金属有机骨架（MOF）MIL-88A 基于药物纳米载体的连接物）的定量分析。（MIL 是 Institut Lavoisier 材料的缩写）。在液-液萃取程序后，通过简单准确的高效液相色谱（HPLC）方法并结合光电二极管阵列检测器（PDA），使用氨基水杨酸作为内标（IS）和梯度洗脱来测定富马酸的浓度。富马酸的回收率对于尿（对于 0.05 和 1μgml（-1）的浓度，分别为 89%和 92%）和肝、脾组织为 90%，与 IS 相比，所有情况下均超过 89%。线性度保持在 0.05 至 1μgml（-1）和 0.5 至 10μgg（-1）之间，分别为尿和组织中的富马酸。该方法的检测限为每次注射 0.01μg。该方法最终允许在静脉注射 MIL-88A 纳米粒子后在大鼠尿和组织样品中定量分析富马酸。
