UMR 8180 CNRS, Institut Lavoisier, Université de Versailles Saint Quentin en Yvelines, 45 Avenue des Etats Unis, 78035 Versailles, France.
J Pharm Biomed Anal. 2012 Aug-Sep;67-68:98-103. doi: 10.1016/j.jpba.2012.04.014. Epub 2012 Apr 20.
Tetramethyl-terephthalate (TMT) is the constitutive linker of the flexible porous iron(III) carboxylate Metal Organic Framework (MOF) MIL-88B_4CH₃ based drug nanocarrier (MIL stands for Material from Institut Lavoisier). A method for the determination of the concentration of tetramethyl-terephthalic acid has been developed in different biological rat matrices (liver, spleen and urine) using a liquid-liquid phase extraction and high-performance liquid chromatography (HPLC) coupled to photodiode array detection with 4-aminosalicylic acid as internal standard. The extraction conditions of TMT have been varied from urine to tissue depending on the complexity of the biological matrices. The chromatographic separation was performed with a gradient elution. In all matrices, the limits of detection and quantification of TMT was 0.01 and 0.05 μg ml⁻¹, respectively. The recovery of the TMT reached 86, 89 and 97% for urine, spleen and liver tissues, respectively. The linearity of the calibration curves in urine and tissues was satisfactory in all cases as evidenced by correlation coefficients >0.990. The within-day and between-day precisions were <15% (n=6) and the accuracy ranged in all cases between 86 and 103%. This method has finally allowed the quantification of TMT in rat urine and in tissue samples of rats administered intravenously with iron(III) tetramethyltherepthalate MIL-88B_4CH₃ nanoparticles.
四甲基对苯二甲酸（TMT）是柔性多孔铁（III）羧酸金属有机骨架（MOF） MIL-88B_4CH₃ 基于药物纳米载体的结构连接剂（MIL 代表来自 Institut Lavoisier 的材料）。开发了一种在不同的生物大鼠基质（肝、脾和尿）中测定四甲基对苯二甲酸浓度的方法，该方法采用液-液萃取和高效液相色谱（HPLC）与光电二极管阵列检测联用，以 4-氨基水杨酸为内标。根据生物基质的复杂程度，TMT 的萃取条件从尿液到组织有所不同。色谱分离采用梯度洗脱。在所有基质中，TMT 的检测限和定量限分别为 0.01 和 0.05 μg ml⁻¹。TMT 的回收率在尿液、脾脏和肝脏组织中分别达到 86%、89%和 97%。在所有情况下，尿液和组织中的校准曲线线性都令人满意，相关系数均>0.990。日内和日间精密度均<15%（n=6），准确度在所有情况下均在 86%至 103%之间。该方法最终允许定量测定静脉注射铁（III）四甲基对苯二甲酸 MIL-88B_4CH₃ 纳米粒子的大鼠尿和组织样本中的 TMT。
