School of Chemistry and Chemical Engineering, Southeast University, Sipeilou 2#, Nanjing 211189, PR China.
Biosens Bioelectron. 2011 Oct 15;28(1):314-9. doi: 10.1016/j.bios.2011.07.045. Epub 2011 Jul 22.
Simultaneous detection of multianalytes associated with a particular cancer is beneficial for disease diagnosis. Here, a facile immunosensing strategy was designed to allow simultaneous electrochemical detection of dual proteins, in a single run. CdSe and PbS water-soluble quantum dots (QDs) were prepared and coated on monodisperse silica nanoparticles as labels for proteins detection. Rabbit immunoglobulin G antigen (IgG) and carcinoembryonic antigen (CEA) were chosen as model proteins for analysis. After a typical sandwich immunoassay, CdSe and PbS QDs labels were introduced onto the Au substrates' surface, which were then dissolved and could be simultaneously monitored by square-wave-voltammetric (SWV) stripping measurements. Under selected conditions, IgG and CEA could be assayed in the ranges of 0.05-40 ng mL(-1) and 0.05-25 ng mL(-1), respectively. The proposed method possessed high sensitivity, good precision, and satisfactory reproducibility and regeneration.
同时检测与特定癌症相关的多种分析物有利于疾病诊断。在这里，设计了一种简便的免疫传感策略，可在单次运行中同时电化学检测两种蛋白质。制备了 CdSe 和 PbS 水溶性量子点 (QD)，并将其涂覆在单分散二氧化硅纳米颗粒上作为蛋白质检测的标记物。兔免疫球蛋白 G 抗原 (IgG) 和癌胚抗原 (CEA) 被选为分析的模型蛋白。在典型的三明治免疫分析后，将 CdSe 和 PbS QD 标记物引入 Au 基底表面，然后将其溶解，并可通过方波伏安法 (SWV) 剥离测量进行同时监测。在选定的条件下，IgG 和 CEA 的检测范围分别为 0.05-40ngmL(-1) 和 0.05-25ngmL(-1)。该方法具有高灵敏度、良好的精密度、令人满意的重现性和再生性。
