Centro de Investigación en Ciencia Aplicada y Tecnología de Avanzada, IPN, Legaria 694, México, DF, Mexico.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Nov;82(1):283-9. doi: 10.1016/j.saa.2011.07.050. Epub 2011 Jul 23.
Lanreotide, a somatostatin analogue peptide used for peptide receptor mediated therapy in metastatic neuroendocrine tumors, was used as capping agent of gold nanoparticles (GNPs) obtained by citrate reduction method. The displacement of the citrate groups from the GNPs surface by Lanreotide (LAN) molecules was evidenced by infrared and Raman spectra. The nanoparticles system, Au@LAN, was also characterized from HRTEM (High-Resolution Transmission Electron Microscopy) and Z-contrast images, UV-vis and EDS spectra. The stability on aging in water solution of the composite is discussed from the UV-vis spectra. The affinity constant of Au@LAN conjugate, calculated from Capillary Zone Electrophoresis data, was found to be 0.52. All the experimental evidence supports that the gold nanoparticles are effectively capped by the Lanreotide molecules through relatively strong covalent interactions. This result opens the possibility of combining the optical properties of gold nanoparticles and of Lanreotide molecule to form a bifunctional system for potential biomedical applications.
兰瑞肽是一种生长抑素类似肽，用于转移性神经内分泌肿瘤的肽受体介导治疗，被用作通过柠檬酸盐还原法获得的金纳米粒子（GNPs）的封端剂。通过红外和拉曼光谱证实了兰瑞肽（LAN）分子从 GNPs 表面取代柠檬酸盐基团。Au@LAN 纳米粒子系统还通过高分辨率透射电子显微镜（HRTEM）和 Z 对比度图像、紫外可见和 EDS 光谱进行了表征。从紫外可见光谱讨论了复合在水溶液中的老化稳定性。从毛细管区带电泳数据计算得到的 Au@LAN 缀合物的亲和常数为 0.52。所有实验证据表明，金纳米粒子通过相对较强的共价相互作用被兰瑞肽分子有效地封端。这一结果为将金纳米粒子的光学性质与兰瑞肽分子相结合，形成用于潜在生物医学应用的双功能系统开辟了可能性。
