Analytical Chemistry Department, University of Barcelona, Avda. Diagonal 647, E-08028 Barcelona, Spain.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Sep 1;879(25):2601-10. doi: 10.1016/j.jchromb.2011.07.018. Epub 2011 Jul 20.
The aim of this study was to develop and validate an analytical method to simultaneously determine European Union-regulated β-lactams (penicillins and cephalosporins) and quinolones in cow milk. The procedure involves a new solid phase extraction (SPE) to clean-up and pre-concentrate the three series of antibiotics before analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). LC-MS/MS and UPLC-MS/MS techniques were also compared. The method was validated according to the Directive 2002/657/EC and subsequently applied to 56 samples of raw cow milk supplied by the Laboratori Interprofessional Lleter de Catalunya (ALLIC) (Laboratori Interprofessional Lleter de Catalunya, Control Laboratory Interprofessional of Milk of Catalunya).
本研究旨在开发和验证一种分析方法，以同时测定牛奶中的欧盟监管的β-内酰胺类抗生素（青霉素类和头孢菌素类）和喹诺酮类药物。该方法包括一种新的固相萃取（SPE），用于在通过液相色谱-串联质谱法（LC-MS/MS）和超高效液相色谱-串联质谱法（UPLC-MS/MS）分析之前，对这三类抗生素进行净化和预浓缩。还比较了 LC-MS/MS 和 UPLC-MS/MS 技术。该方法根据指令 2002/657/EC 进行了验证，随后应用于 Laboratori Interprofessional Lleter de Catalunya（ALLIC）（Laboratori Interprofessional Lleter de Catalunya，Catalonia 牛奶的跨行业实验室）提供的 56 份生牛乳样品中。
