Institute of Quality Standards and Testing Technology for Agricultural Products, Xinjiang Academy of Agricultural Science, Urumqi 830091, China.
Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, Beijing 100081, China.
Molecules. 2023 Apr 26;28(9):3738. doi: 10.3390/molecules28093738.
As a non-traditional sample matrix, feather samples can be used to effectively monitor antibiotic addition and organismal residue levels in poultry feeding. Therefore, an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to simultaneously determine the residue levels of 26 quinolones in poultry feathers. The feather samples were extracted by sonication with a 1% formic acid and acetonitrile mixture in a water bath at 50 °C for 30 min, purified by the adsorption of multiple matrix impurities, dried with nitrogen, redissolved, and analyzed by UPLC-MS/MS. The linearity, limit of detection (LOD), limit of quantification (LOQ), recovery and precision were calculated. The 26 antibiotics demonstrated good linearity in the linear range. The recoveries and coefficients of variation were 78.9-110% and <13.7% at standard spiked levels of 10, 100 and 200 μg/kg, respectively. The LOD and LOQ were 0.12-1.31 and 0.96-2.60 μg/kg, respectively. The method also successfully identified quinolone residues in 50 poultry feather samples. The results showed that quinolones can accumulate and stabilize for a certain period of time after transferring from the body to the feathers of poultry.
作为一种非传统的样本基质，羽毛样本可用于有效监测禽类饲养中抗生素的添加和生物体残留水平。因此，建立了一种超高效液相色谱-串联质谱法（UPLC-MS/MS），用于同时检测禽羽毛中 26 种喹诺酮类药物的残留水平。将羽毛样本在 50°C 的水浴中用 1%甲酸和乙腈混合物超声提取 30 分钟，用多基质杂质吸附法进行净化，氮气干燥，重新溶解，然后用 UPLC-MS/MS 进行分析。计算了线性、检测限（LOD）、定量限（LOQ）、回收率和精密度。26 种抗生素在线性范围内表现出良好的线性关系。在 10、100 和 200μg/kg 的标准添加水平下，回收率和变异系数分别为 78.9-110%和 <13.7%。LOD 和 LOQ 分别为 0.12-1.31 和 0.96-2.60μg/kg。该方法还成功地鉴定了 50 个禽羽毛样本中的喹诺酮残留。结果表明，喹诺酮类药物从体内转移到禽羽毛后，可在一定时间内积累并稳定存在。
