Forrest G L, Akman S, Krutzik S, Paxton R J, Sparkes R S, Doroshow J, Felsted R L, Glover C J, Mohandas T, Bachur N R
Division of Biology, Beckman Research Institute at the City of Hope, Duarte, CA 91010.
Biochim Biophys Acta. 1990 Apr 6;1048(2-3):149-55. doi: 10.1016/0167-4781(90)90050-c.
Carbonyl reductase (EC 1.1.1.184) belongs to the group of enzymes called aldo-keto reductases. It is a NADPH-dependent cytosolic protein with specificity for many carbonyl compounds including the antitumor anthracycline antibiotics, daunorubicin and doxorubicin. Human carbonyl reductase was cloned from a breast cancer cell line (MCF-7). The cDNA clone contained 1219 base paires with an open reading frame corresponding to 277 amino acids encoding a protein of Mr 30,375. Southern analysis of genomic DNA digested with several restriction enzymes and analyzed by hybridization with a labeled cDNA probe indicated that carbonyl reductase is probably coded by a single gene and does not belong to a family of structurally similar enzymes. Southern analysis of 17 mouse/human somatic cell hybrids showed that carbonyl reductase is located on chromosome 21. Carbonyl reductase mRNA could be induced 3-4-fold in 24 h with 10 microM 2,(3)-t-butyl-4-hydroxyanisole (BHA), beta-naphthoflavone or Sudan 1.
羰基还原酶（EC 1.1.1.184）属于醛酮还原酶类。它是一种依赖NADPH的胞质蛋白，对许多羰基化合物具有特异性，包括抗肿瘤蒽环类抗生素柔红霉素和阿霉素。人羰基还原酶是从乳腺癌细胞系（MCF - 7）中克隆出来的。该cDNA克隆包含1219个碱基对，其开放阅读框对应于277个氨基酸，编码一个分子量为30375的蛋白质。用几种限制性酶消化基因组DNA并与标记的cDNA探针杂交进行Southern分析表明，羰基还原酶可能由单个基因编码，不属于结构相似的酶家族。对17个小鼠/人类体细胞杂种进行的Southern分析表明，羰基还原酶位于21号染色体上。用10微摩尔/升的2，（3）-叔丁基-4-羟基茴香醚（BHA）、β-萘黄酮或苏丹1在24小时内可使羰基还原酶mRNA诱导3 - 4倍。
