利用孢子表面展示的 N-乙酰-D-氨基葡萄糖醛酸醛缩酶从 N-乙酰-D-氨基葡萄糖合成 N-乙酰-D-神经氨酸。
Chemoenzymatic synthesis of N-acetyl-D-neuraminic acid from N-acetyl-D-glucosamine by using the spore surface-displayed N-acetyl-D-neuraminic acid aldolase.
机构信息
State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
出版信息
Appl Environ Microbiol. 2011 Oct;77(19):7080-3. doi: 10.1128/AEM.05601-11. Epub 2011 Aug 5.
Abstract
Chemoenzymatic synthesis of N-acetyl-d-neuraminic acid from N-acetyl-d-glucosamine using the spore surface-displayed N-acetyl-d-neuraminic acid aldolase at a high concentration (53.9 g liter(-1)) was achieved in this study. Thus, displaying a target enzyme on the surface of spores might be an alternative for integration of biocatalytic conversion into chemical synthesis.
摘要
本研究在 53.9 g/L 的高浓度下，利用孢子表面展示的 N-乙酰-D-氨基葡萄糖醛酸醛缩酶，从 N-乙酰-D-氨基葡萄糖合成 N-乙酰-D-神经氨酸。因此，在孢子表面展示目标酶可能是将生物催化转化整合到化学合成中的一种替代方法。
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