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本文引用的文献

1
Production of N-acetyl-D-neuraminic acid by use of an efficient spore surface display system.利用高效的孢子表面展示系统生产 N-乙酰-D-神经氨酸。
Appl Environ Microbiol. 2011 May;77(10):3197-201. doi: 10.1128/AEM.00151-11. Epub 2011 Mar 25.
2
Display of recombinant proteins on Bacillus subtilis spores, using a coat-associated enzyme as the carrier.利用衣壳相关酶作为载体在枯草芽孢杆菌孢子上展示重组蛋白。
Appl Environ Microbiol. 2010 Sep;76(17):5926-33. doi: 10.1128/AEM.01103-10. Epub 2010 Jul 2.
3
Biotechnological production and applications of N-acetyl-D-neuraminic acid: current state and perspectives.N-乙酰-D-神经氨酸的生物技术生产及应用：现状与展望。
Appl Microbiol Biotechnol. 2010 Jul;87(4):1281-9. doi: 10.1007/s00253-010-2700-6. Epub 2010 Jun 8.
4
An efficient method for N-acetyl-D-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system.利用具有安全温度诱导系统的偶联细菌细胞生产 N-乙酰-D-神经氨酸的有效方法。
Appl Microbiol Biotechnol. 2010 Mar;86(2):481-9. doi: 10.1007/s00253-009-2302-3. Epub 2009 Nov 4.
5
Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins.利用作为双标签融合蛋白过表达的两种连续酶生产N-乙酰-D-神经氨酸。
BMC Biotechnol. 2009 Jul 9;9:63. doi: 10.1186/1472-6750-9-63.
6
Display of proteins on Bacillus subtilis endospores.蛋白质在枯草芽孢杆菌芽孢上的展示。
Cell Mol Life Sci. 2009 Oct;66(19):3127-36. doi: 10.1007/s00018-009-0067-6. Epub 2009 Jun 25.
7
Inhibition of Bacillus anthracis spore outgrowth by nisin.乳链菌肽对炭疽芽孢杆菌芽孢萌发的抑制作用。
Antimicrob Agents Chemother. 2008 Dec;52(12):4281-8. doi: 10.1128/AAC.00625-08. Epub 2008 Sep 22.
8
Functional expression in Bacillus subtilis of mammalian NADPH-cytochrome P450 oxidoreductase and its spore-display.哺乳动物NADPH-细胞色素P450氧化还原酶在枯草芽孢杆菌中的功能表达及其孢子展示
Protein Expr Purif. 2009 Jan;63(1):5-11. doi: 10.1016/j.pep.2008.07.004. Epub 2008 Jul 19.
9
Production of N-acetyl-D-neuraminic acid by recombinant whole cells expressing Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase and Escherichia coli N-acetyl-D-neuraminic acid lyase.通过表达鱼腥藻CH1 N-乙酰-D-葡萄糖胺2-表异构酶和大肠杆菌N-乙酰-D-神经氨酸裂解酶的重组全细胞生产N-乙酰-D-神经氨酸
J Biotechnol. 2007 May 1;129(3):453-60. doi: 10.1016/j.jbiotec.2007.01.027. Epub 2007 Feb 9.
10
Transgalactosylation in a water-solvent biphasic reaction system with beta-galactosidase displayed on the surfaces of Bacillus subtilis spores.在枯草芽孢杆菌孢子表面展示的β-半乳糖苷酶参与的水-溶剂双相反应体系中的转半乳糖基化反应。
Appl Environ Microbiol. 2007 Apr;73(7):2251-6. doi: 10.1128/AEM.01489-06. Epub 2006 Dec 22.

利用孢子表面展示的 N-乙酰-D-氨基葡萄糖醛酸醛缩酶从 N-乙酰-D-氨基葡萄糖合成 N-乙酰-D-神经氨酸。

Chemoenzymatic synthesis of N-acetyl-D-neuraminic acid from N-acetyl-D-glucosamine by using the spore surface-displayed N-acetyl-D-neuraminic acid aldolase.

机构信息

State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

出版信息

Appl Environ Microbiol. 2011 Oct;77(19):7080-3. doi: 10.1128/AEM.05601-11. Epub 2011 Aug 5.

DOI:10.1128/AEM.05601-11
PMID:21821765
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3187080/
Abstract

Chemoenzymatic synthesis of N-acetyl-d-neuraminic acid from N-acetyl-d-glucosamine using the spore surface-displayed N-acetyl-d-neuraminic acid aldolase at a high concentration (53.9 g liter(-1)) was achieved in this study. Thus, displaying a target enzyme on the surface of spores might be an alternative for integration of biocatalytic conversion into chemical synthesis.

摘要

本研究在 53.9 g/L 的高浓度下，利用孢子表面展示的 N-乙酰-D-氨基葡萄糖醛酸醛缩酶，从 N-乙酰-D-氨基葡萄糖合成 N-乙酰-D-神经氨酸。因此，在孢子表面展示目标酶可能是将生物催化转化整合到化学合成中的一种替代方法。