Kim Junehyung, Schumann Wolfgang
Department of Chemical Engineering, Dong-A University, Busan, Republic of Korea.
Cell Mol Life Sci. 2009 Oct;66(19):3127-36. doi: 10.1007/s00018-009-0067-6. Epub 2009 Jun 25.
The targeting and anchoring of heterologous proteins and peptides to the outer surface of bacteriophages and cells is becoming increasingly important, and has been employed as a tool for fundamental and applied research in microbiology, molecular biology, vaccinology, and biotechnology. Less known are endospores or spores produced by some Gram-positive species. Spores of Bacillus subtilis are surrounded by a spore coat on their outside, and a few proteins have been identified being located on the outside layer and have been successfully used to immobilize antigens and some other proteins and enzymes. The major advantage of spores over the other published systems is their synthesis within the cytoplasm of the bacterial cell. Therefore, any heterologous protein to be anchored on the outside does not have to cross any membrane. Furthermore, spores are extremely resistant against high temperature, irradiation and many chemicals, and can be stored for many years at room temperature.
将异源蛋白质和肽靶向并锚定到噬菌体和细胞的外表面正变得越来越重要，并已被用作微生物学、分子生物学、疫苗学和生物技术基础研究与应用研究的工具。一些革兰氏阳性菌产生的内生孢子或芽孢则鲜为人知。枯草芽孢杆菌的芽孢外部被一层芽孢衣所包围，并且已经鉴定出一些位于外层的蛋白质，并已成功用于固定抗原以及其他一些蛋白质和酶。与其他已发表的系统相比，芽孢的主要优势在于它们在细菌细胞的细胞质内合成。因此，任何要锚定在外表面的异源蛋白质都无需穿过任何膜。此外，芽孢对高温、辐射和许多化学物质具有极强的抵抗力，并且可以在室温下储存多年。
