Zhang Nianzhi, Qi Jianxun, Pan Xiaocheng, Chen ZhaoSan, Li Xin, Gao Feng, Xia Chun
Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):888-91. doi: 10.1107/S1744309111020392. Epub 2011 Jul 19.
CD8αα homodimers or CD8αβ heterodimers form on the T-cell surface, where they are essential as co-receptors for MHC class I molecules in activation of the CTL response. To date, swine have been found to show the highest percentage of lymphocytes with surface expression of CD8α. Crystallographic analysis of swine CD8α (sCD8α) to 1.8 Å resolution revealed that the crystals belonged to space group P3(2)21, with unit-cell parameters a = 80.97, b = 80.97, c = 95.19 Å. The Matthews coefficient and the solvent content were calculated to be 3.23 Å(3) Da(-1) and 61.89%, respectively. These results may aid further structural and functional analyses of sCD8α.
CD8αα同型二聚体或CD8αβ异型二聚体在T细胞表面形成，在那里它们作为MHC I类分子的共受体对CTL反应的激活至关重要。迄今为止，已发现猪的淋巴细胞表面表达CD8α的比例最高。对猪CD8α（sCD8α）进行分辨率为1.8 Å的晶体学分析，结果显示晶体属于空间群P3(2)21，晶胞参数a = 80.97、b = 80.97、c = 95.19 Å。计算得出的马修斯系数和溶剂含量分别为3.23 Å(3) Da(-1)和61.89%。这些结果可能有助于对sCD8α进行进一步的结构和功能分析。
