Pan Xiaocheng, Qi Jianxun, Zhang Nianzhi, Li Qirun, Yin Chunsheng, Chen Rong, Gao Feng, Xia Chun
Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Bejing 100094, People's Republic of China.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 May 1;67(Pt 5):568-71. doi: 10.1107/S174430911100741X. Epub 2011 Apr 27.
In order to illustrate the structure of the swine MHC class I (SLA-I) molecule and to evaluate the cytotoxic T lymphocyte (CTL) response against porcine reproductive and respiratory syndrome virus (PRRSV), the ternary complex of the SLA-I molecule termed SLA-1*1502 with β(2)-microglobulin and the CTL epitope TMPPGFELY (PRRSV-NSP9(TY9)) derived from PRRSV nonstructural protein 9 (residues 198-206) was assembled and crystallized. The crystal diffracted X-rays to 2.2 Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 66.1, b = 74.1, c = 98.6 Å; it contained one molecule in the asymmetric unit. The Matthews coefficient and the solvent content were calculated to be 2.74 Å(3) Da(-1) and 55.17%, respectively. The results will be helpful in obtaining insight into the structural basis of the presentation of viral epitopes by SLA-I.
为了阐明猪主要组织相容性复合体I类（SLA-I）分子的结构，并评估细胞毒性T淋巴细胞（CTL）对猪繁殖与呼吸综合征病毒（PRRSV）的应答，我们构建并结晶了SLA-I分子SLA-1*1502与β2-微球蛋白以及源自PRRSV非结构蛋白9（第198-206位氨基酸）的CTL表位TMPPGFELY（PRRSV-NSP9(TY9)）形成的三元复合物。该晶体的X射线衍射分辨率达到2.2 Å，属于空间群P2(1)2(1)2(1)，晶胞参数为a = 66.1、b = 74.1、c = 98.6 Å；不对称单元中含有一个分子。计算得到的马修斯系数和溶剂含量分别为2.74 Å(3) Da(-1)和55.17%。这些结果将有助于深入了解SLA-I呈递病毒表位的结构基础。
