Wang Zhenbao, Chen Rong, Tariq Mansoor, Jiang Bo, Chen Zhaosan, Xia Chun
Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
Acta Crystallogr F Struct Biol Commun. 2014 Jun;70(Pt 6):742-6. doi: 10.1107/S2053230X14008838. Epub 2014 May 10.
In order to clarify the structural characteristics of the bovine MHC class I molecule (BoLA-I) complexed with CD8αα (CD8αα-BoLA-I), bovine CD8αα, BoLA-I (BoLA-202201) and β2m were expressed and purified, and were then assembled with a peptide derived from Foot-and-mouth disease virus (FMDV-VP1YY9) and crystallized. The crystal diffracted to 1.7 Å resolution and belonged to space group P21, with unit-cell parameters a=53.9, b=103.8, c=61.8 Å, α=γ=90, β=96°. The asymmetric unit contained one complex, with a Matthews coefficient of 2.41 Å3 Da(-1) and a solvent content of 48.9%. The rotation-function Z-score and translation-function Z-score for molecular replacement were 3.4 and 8.9, respectively. In addition, SDS-PAGE analysis of CD8αα-BoLA-I crystals showed three bands corresponding to the molecular weights of BoLA-I heavy chain, β2m and CD8α. The structure of the CD8αα-BoLA-I complex should be helpful in obtaining insight into the interaction between bovine CD8αα and MHC class I molecules. Structure determination of BoLA-202201-FMDV-VP1YY9 will be useful in the design of vaccines for foot-and-mouth disease.
为阐明与CD8αα复合的牛MHC I类分子（BoLA-I）（CD8αα-BoLA-I）的结构特征，表达并纯化了牛CD8αα、BoLA-I（BoLA-202201）和β2m，然后与源自口蹄疫病毒（FMDV-VP1YY9）的肽组装并结晶。该晶体衍射分辨率达到1.7 Å，属于空间群P21，晶胞参数为a = 53.9、b = 103.8、c = 61.8 Å，α = γ = 90°，β = 96°。不对称单元包含一个复合物，马修斯系数为2.41 Å3 Da-1，溶剂含量为48.9%。分子置换的旋转函数Z值和翻译函数Z值分别为3.4和8.9。此外，CD8αα-BoLA-I晶体的SDS-PAGE分析显示出三条对应于BoLA-I重链、β2m和CD8α分子量的条带。CD8αα-BoLA-I复合物的结构应有助于深入了解牛CD8αα与MHC I类分子之间的相互作用。BoLA-202201-FMDV-VP1YY9的结构测定将有助于口蹄疫疫苗的设计。
