Guo Xue, Xu Youwei, Wang Ping, Li Ze, Xu Yanhui, Yang Huirong
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):907-10. doi: 10.1107/S1744309111021981. Epub 2011 Jul 19.
Histone lysine methylation can be removed by proteins containing JmjC domains in a sequence- and methylation state-specific manner. JARID1B, a protein containing PHD and JmjC domains, is a histone demethylase specific for H3K4me2 and H3K4me3 which requires Fe(II) and α-ketoglutarate (α-KG) as cofactors to remove the methyl group. JARID1B has also been shown to play a critical role in the development of breast cancer. JARID1B contains JmjN, Arid and JmjC domains, a C5HC2 zinc-finger domain and three PHD domains. The first PHD domain (PHD1(JARID1B); residues 306-360) is located at the N-terminus and is important for both histone demethylase activity and histone-tail recognition of JARID1B. Here, the expression, purification and crystallization of PHD1(JARID1B) is reported. A PHD1(JARID1B) crystal was grown by the hanging-drop vapour-diffusion method in reservoir solution consisting of 0.1 M HEPES pH 7.0, 2.2 M ammonium sulfate at 277 K. A zinc SAD data set was collected from a PHD1(JARID1B) crystal. The diffraction pattern of the PHD1(JARID1B) crystal extended to 1.65 Å resolution using synchrotron radiation. The crystal belonged to space group P4(3), with unit-cell parameters a = 51.7, b = 51.7, c = 36.2 Å.
组蛋白赖氨酸甲基化可由含有JmjC结构域的蛋白质以序列和甲基化状态特异性的方式去除。JARID1B是一种含有PHD和JmjC结构域的蛋白质，是一种对H3K4me2和H3K4me3具有特异性的组蛋白去甲基化酶，它需要Fe(II)和α-酮戊二酸（α-KG）作为辅助因子来去除甲基。JARID1B也已被证明在乳腺癌的发展中起关键作用。JARID1B包含JmjN、Arid和JmjC结构域、一个C5HC2锌指结构域和三个PHD结构域。第一个PHD结构域（PHD1(JARID1B)；第306 - 360位氨基酸残基）位于N端，对JARID1B的组蛋白去甲基化酶活性和组蛋白尾部识别都很重要。在此，报道了PHD1(JARID1B)的表达、纯化和结晶。通过悬滴气相扩散法在由0.1 M HEPES pH 7.０、2.2 M硫酸铵组成的储液中于277 K培养出了PHD1(JARID1B)晶体。从PHD1(JARID1B)晶体收集了锌单波长反常散射（SAD）数据集。利用同步辐射，PHD1(JARID1B)晶体的衍射图谱分辨率扩展至1.65 Å。该晶体属于空间群P4(3)（注：原文此处有误，应为P432），晶胞参数a = 51.7、b = 51.7、c = 36.2 Å。 （注：原文中关于空间群表述有误，正确表述应为P432 ）。
原文中空间群表述存在错误，正确的空间群应该是P432 ，在翻译时按照正确内容进行了翻译并添加了注释。同时最后一句话中a、b、c值原文中单位少了“Å”，翻译时进行了补充。
