Ge Honghua, Xu Peisong, Xu Ying, Fang Zemin, Xiao Yazhong
Modern Experiment Technology Center and School of Life Sciences, Anhui University, Hefei, People's Republic of China.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):956-8. doi: 10.1107/S1744309111024912. Epub 2011 Jul 27.
Laccases are members of the blue multi-copper oxidase family that can oxidize a wide range of aromatic compounds. A new bacterial laccase (Lac15) has recently been obtained from a marine microbial metagenome from the South China Sea and characterized. In this work, recombinant Lac15 was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. An X-ray diffraction data set was collected to 2.2 Å resolution. The crystal belonged to space group C121, with unit-cell parameters a = 123.41, b = 91.36, c = 86.157 Å, β = 112.10°.
漆酶是蓝色多铜氧化酶家族的成员，能够氧化多种芳香族化合物。最近从中国南海海洋微生物宏基因组中获得了一种新的细菌漆酶（Lac15）并对其进行了表征。在这项工作中，重组Lac15在大肠杆菌中过量表达，采用悬滴气相扩散法进行纯化和结晶。收集到了分辨率为2.2 Å的X射线衍射数据集。该晶体属于空间群C121，晶胞参数为a = 123.41、b = 91.36、c = 86.157 Å，β = 112.10°。
