从蛇足石杉中分离得到的一种产吖啶酮的新型多功能III型聚酮合酶的结晶及初步晶体学分析。
Crystallization and preliminary crystallographic analysis of an acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata.
作者信息
Morita Hiroyuki, Kondo Shin, Kato Ryohei, Wanibuchi Kiyofumi, Noguchi Hiroshi, Sugio Shigetoshi, Abe Ikuro, Kohno Toshiyuki
机构信息
Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt 7):576-8. doi: 10.1107/S1744309107026164. Epub 2007 Jun 11.
Abstract
Polyketide synthase 1 (PKS1) from Huperzia serrata is a plant-specific type III polyketide synthase that shows an unusually versatile catalytic potential, producing various aromatic tetraketides, including chalcones, benzophenones, phlorogulucinols and acridones. Recombinant H. serrata PKS1 expressed in Escherichia coli was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 73.3, b = 85.0, c = 137.7 A, alpha = beta = gamma = 90.0 degrees. Diffraction data were collected to 2.0 A resolution using synchrotron radiation at BL24XU of SPring-8.
摘要
来自蛇足石杉的聚酮合酶1（PKS1）是一种植物特异性III型聚酮合酶，具有异常多样的催化潜力，可产生各种芳香族四酮类化合物，包括查耳酮、二苯甲酮、间苯三酚和吖啶酮。在大肠杆菌中表达的重组蛇足石杉PKS1采用悬滴气相扩散法进行结晶。晶体属于空间群I222或I2(1)2(1)2(1)，晶胞参数a = 73.3，b = 85.0，c = 137.7 Å，α = β = γ = 90.0°。使用SPring-8的BL24XU同步辐射将衍射数据收集到2.0 Å分辨率。
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