Konishi Atsushi, Nemoto Daisuke, Yasukawa Kiyoshi, Inouye Kuniyo
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan.
Biosci Biotechnol Biochem. 2011;75(8):1618-20. doi: 10.1271/bbb.110238. Epub 2011 Aug 7.
Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63-kDa α subunit and a 95-kDa β subunit. In this study, we explored the role of the interaction between the α and β subunits on AMV RT stability. The recombinant AMV RT α subunit was expressed in insect cells and purified. It exhibited lower thermal stability than the native AMV RT αβ heterodimer. Unlike the αβ heterodimer, the α subunit was not stabilized by template-primer. These results suggest that interaction between the α and β subunits is important for AMV RT stability.
禽成髓细胞瘤病毒逆转录酶（AMV RT）是一种异源二聚体，由一个63 kDa的α亚基和一个95 kDa的β亚基组成。在本研究中，我们探讨了α亚基和β亚基之间的相互作用对AMV RT稳定性的作用。重组AMV RT α亚基在昆虫细胞中表达并纯化。它表现出比天然AMV RT αβ异源二聚体更低的热稳定性。与αβ异源二聚体不同，α亚基不能被模板引物稳定。这些结果表明α亚基和β亚基之间的相互作用对AMV RT稳定性很重要。
