School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan.
Life Science Research Laboratory, Tosoh Corp., Ayase, Kanagawa, Japan.
Biosci Biotechnol Biochem. 2021 May 25;85(6):1464-1467. doi: 10.1093/bbb/zbab057.
The α subunit of avian myeloblastosis virus reverse transcriptase (AMV-RT) is generated from the β-subunit by proteolysis, and the αβ heterodimer represents the active form. The codon-optimized gene was expressed in Escherichia coli, and an active αβ heterodimer was generated. The RNA amplification activity of the purified recombinant AMV-RT αβ heterodimer was similar to that of the native one.
禽髓细胞瘤病毒逆转录酶（AMV-RT）的α亚单位通过蛋白水解作用从β亚单位生成，αβ异二聚体代表活性形式。经过密码子优化的基因在大肠杆菌中表达，并生成了有活性的αβ异二聚体。纯化的重组 AMV-RT αβ异二聚体的 RNA 扩增活性与天然的相似。
