Gasper Gerald L, Takahashi Lynelle K, Zhou Jia, Ahmed Musahid, Moore Jerry F, Hanley Luke
Department of Chemistry, University of Illinois at Chicago, 845 W. Taylor Street (m/c 111), Chicago, IL 60607, USA.
Nucl Instrum Methods Phys Res A. 2011 Sep 1;649(1):222-224. doi: 10.1016/j.nima.2010.12.024.
Vacuum and extreme ultraviolet radiation from 8 - 24 eV generated at a synchrotron was used to postionize laser desorbed neutrals of antibiotic-treated biofilms and a modified fullerene using laser desorption postionization mass spectrometry (LDPI-MS). Results show detection of the parent ion, various fragments, and extracellular material from biofilms using LDPI-MS with both vacuum and extreme ultraviolet photons. Parent ions were observed for both cases, but extreme ultraviolet photons (16 - 24 eV) induced more fragmentation than vacuum ultraviolet (8 - 14 eV) photons.
利用同步加速器产生的8 - 24电子伏特的真空和极紫外辐射，通过激光解吸后电离质谱法（LDPI-MS）对经抗生素处理的生物膜和改性富勒烯的激光解吸中性粒子进行后电离。结果表明，使用真空和极紫外光子的LDPI-MS能够检测到生物膜的母离子、各种碎片和细胞外物质。两种情况下均观察到了母离子，但极紫外光子（16 - 24电子伏特）比真空紫外光子（8 - 14电子伏特）诱导产生了更多的碎片。
