Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, Seocheon-dong, Giheung-gu, Yongin, Gyeonggi-do 446-701, Republic of Korea.
Mediators Inflamm. 2011;2011:945123. doi: 10.1155/2011/945123. Epub 2011 May 24.
The effects of dietary protein and fat on renal function-related blood and urine parameters, such as albumin, urinary protein,and inflammatory cytokines were investigated in adriamycin- (ADR) induced nephrotic syndrome rats. ADR (2 mg/kg BW) was injected i.p. weekly for six weeks to develop nephrotic syndrome; thereafter rats were fed low-protein/high-fat (LPHF) or high-protein/low-fat (HPLF) diets for five weeks. Renal function-related blood and urine parameters were measured before and after dietary intervention. Serum levels of albumin, TG, and creatinine were significantly higher in the LPHF group than in the HPLF group. Serum levels of albumin were low and urinary protein excretion protein was high in HPLF group. BUN and UUN levels were higher in the HPLF group than in the LPHF. Urinary excretion of creatinine was significantly higher in the HPLF group than in the LPHF group. Serum inflammatory cytokine levels did not differ between the two groups, however the levels of IL-6, TNF-α, and IL-13 in splenocyte supernatants were significantly higher in the LPHF group than in the HPLF group. We confirmed that protein and fat contents in diet affect renal function-related blood and urine parameters and splenocyte inflammatory cytokine levels in ADR-induced nephrotic syndrome rats.
研究了饮食中的蛋白质和脂肪对阿霉素（ADR）诱导的肾病综合征大鼠肾功能相关的血液和尿液参数（如白蛋白、尿蛋白和炎症细胞因子）的影响。通过每周腹腔注射 2mg/kg BW 的 ADR 6 周来诱导肾病综合征；此后，大鼠分别喂食低蛋白/高脂肪（LPHF）或高蛋白/低脂肪（HPLF）饮食 5 周。在饮食干预前后测量肾功能相关的血液和尿液参数。LPHF 组血清白蛋白、甘油三酯和肌酐水平明显高于 HPLF 组。HPLF 组血清白蛋白水平较低，尿蛋白排泄量较高。HPLF 组 BUN 和 UUN 水平高于 LPHF 组。HPLF 组尿肌酐排泄量明显高于 LPHF 组。两组血清炎症细胞因子水平无差异，但 LPHF 组脾细胞上清液中 IL-6、TNF-α和 IL-13 水平明显高于 HPLF 组。我们证实，饮食中的蛋白质和脂肪含量会影响 ADR 诱导的肾病综合征大鼠的肾功能相关的血液和尿液参数以及脾细胞炎症细胞因子水平。
