Department of Medical Nutrition, Kyung Hee University, Yongin, Korea.
Kidney Blood Press Res. 2012;35(6):438-44. doi: 10.1159/000336361. Epub 2012 Jun 6.
BACKGROUND/AIM: The purpose of the study was to investigate the effects of a high-protein (HP) diet on bone metabolism in rats with adriamycin (ADR)-induced nephrotic syndrome.
Nephrotic syndrome was established by weekly injections of ADR (2 mg/kg, i.p.) for 6 weeks. After a final injection, we confirmed that nephrotic syndrome had developed. Then, the rats were divided into two groups for the dietary treatments, namely the HP diet (30% of calories from protein) and the low-protein (LP) diet (7% of calories from protein), and were fed an isocaloric diet for the following 5 weeks.
Urinary protein and phosphate excretion were significantly greater in the HP diet group than in the LP diet group (p < 0.05). Serum parathyroid hormone and osteocalcin levels were significantly higher and lower, respectively, in the HP diet group (p < 0.05). Femur weight, femur mass index and femur calcium contents were significantly lower in the HP diet group than in the LP diet group (p < 0.05). Bone mineral density was significantly lower in the HP diet group than in the LP diet group (p < 0.05); however, bone mineral content did not differ between the two groups.
We confirmed that an HP diet negatively affects bone mineral metabolism and bone density in ADR-induced nephrotic syndrome rats.
背景/目的：本研究旨在探讨高蛋白（HP）饮食对阿霉素（ADR）诱导的肾病综合征大鼠骨代谢的影响。
通过每周腹腔注射 ADR（2 mg/kg）6 周建立肾病综合征模型。末次注射后，我们确认了肾病综合征的发生。然后，将大鼠分为两组进行饮食治疗，即 HP 饮食（30%的热量来自蛋白质）和 LP 饮食（7%的热量来自蛋白质），并在接下来的 5 周内给予等热量饮食。
HP 饮食组的尿蛋白和磷酸盐排泄量明显高于 LP 饮食组（p < 0.05）。HP 饮食组的血清甲状旁腺激素和骨钙素水平分别明显升高和降低（p < 0.05）。HP 饮食组的股骨重量、股骨质量指数和股骨钙含量明显低于 LP 饮食组（p < 0.05）。HP 饮食组的骨矿物质密度明显低于 LP 饮食组（p < 0.05）；然而，两组的骨矿物质含量没有差异。
我们证实 HP 饮食可负性影响 ADR 诱导的肾病综合征大鼠的骨矿物质代谢和骨密度。
