经典细胞遗传学：核型分析技术。

Classical cytogenetics: karyotyping techniques.

作者信息

Bates Steven E

机构信息

Department of Biology, Beckman Research Institute, City of Hope National Medical Center, Duarte, CA, USA.

出版信息

Methods Mol Biol. 2011;767:177-90. doi: 10.1007/978-1-61779-201-4_13.

DOI:10.1007/978-1-61779-201-4_13

PMID:21822875

Abstract

Classical cytogenetics by karyotyping has been utilized in clinical research laboratories for more than 50 years and remains the key method used in the stem cell laboratory to assess the genetic stability of stem cell cultures. It is currently the most readily accessible method for detecting chromosomal abnormalities in pluripotent stem cell cultures. This chapter will describe (1) how to prepare a culture to maximize the number of metaphase cells, (2) how to prepare slides containing chromosome spreads (3) methods used to stain chromosomes, and (4) how to interpret the cytogenetic report.

摘要

通过核型分析进行的经典细胞遗传学已在临床研究实验室中应用了50多年，并且仍然是干细胞实验室用于评估干细胞培养物遗传稳定性的关键方法。它是目前检测多能干细胞培养物中染色体异常最容易获得的方法。本章将描述：（1）如何制备培养物以最大化中期细胞的数量；（2）如何制备包含染色体铺展的玻片；（3）用于染色体染色的方法；以及（4）如何解读细胞遗传学报告。