Wang Q R, Wolf N S
Experimental Hematology Laboratory, Hunan Medical College, People's Republic of China.
Exp Hematol. 1990 May;18(4):355-9.
In this study it was found that three kinds of cells, fibroblasts, endothelial cells, and macrophages, exist in fibroblastic colony-forming unit (CFU-F) colonies, consistent with the findings of others. However, when low enough bone marrow stromal cell numbers were cultured per dish, pure fibroblast, endothelial, and macrophage colonies were observed. When the cell number cultured in each dish was increased, all colonies were of mixed cell type. This evidence strongly suggests that the macrophages and endothelial cells contained in CFU-F colonies come from locally stimulated growth resulting in contamination and not from a common progenitor with fibroblasts or each other. In some longer term experiments, adipocytes were seen to appear within the colonies of late-passage pure fibroblast cultures.
在本研究中发现，成纤维细胞集落形成单位（CFU-F）集落中存在三种细胞，即成纤维细胞、内皮细胞和巨噬细胞，这与其他人的研究结果一致。然而，当每皿培养的骨髓基质细胞数量足够少时，可观察到纯成纤维细胞、内皮细胞和巨噬细胞集落。当每皿培养的细胞数量增加时，所有集落均为混合细胞类型。这一证据有力地表明，CFU-F集落中所含的巨噬细胞和内皮细胞来自局部刺激生长导致的污染，而非来自与成纤维细胞的共同祖细胞或彼此之间的共同祖细胞。在一些长期实验中，在传代后期的纯成纤维细胞培养物集落中可见脂肪细胞出现。
