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[不同方法提取紫草挥发油的比较]

[Comparison on extraction of volatile oils from Lithospermum erythrorhizon by different methods].

作者信息

Yang Ri-fu, Huang Ping-ping, Qiu Tai-qiu, Fan Xiao-dan

机构信息

College of Science, South China University of Technology, Guangzhou 510640, China.

出版信息

Zhong Yao Cai. 2011 Feb;34(2):289-93.

PMID:21823490
Abstract

OBJECTIVE

To extract the volatile oils from Lithospermum erythrorhizon via ultrasound-enhanced sub-critical water extraction (USWE) and compare with ultrasound-enhanced solvent extraction (USE) and steam distillation extraction (SD).

METHODS

The extraction yield of the volatile oils, the containing components of extract, the effect of scanvenging activities on free radical DPPH and reducing activities as well as the inhibitory on escherichia coli and staphylococcus aureus were investigated.

RESULTS

The extraction yield of volatile oils by USWE, USE and SD were 2.39%, 1.93% and 0.62%, respectively, the extracts by three methods all contained six major components, but the extracts by SD and USE contained more impurities. The inhibitory effect on escherichia coli and staphylococcus aureus of the extract by SD and its reducing action were the best,but those by USWE were the worst.

CONCLUSION

the extraction yield of volatile oils by USWE is the highest, and it contains less impurities based on the worst in reducing power and inhibitory effects.

摘要

目的

采用超声强化亚临界水萃取法(USWE)从紫草中提取挥发油,并与超声强化溶剂萃取法(USE)和水蒸气蒸馏萃取法(SD)进行比较。

方法

考察挥发油的提取率、提取物的成分、对自由基DPPH的清除活性和还原活性以及对大肠杆菌和金黄色葡萄球菌的抑制作用。

结果

USWE、USE和SD法提取挥发油的得率分别为2.39%、1.93%和0.62%,三种方法的提取物均含有六种主要成分,但SD法和USE法的提取物杂质较多。SD法提取物对大肠杆菌和金黄色葡萄球菌的抑制作用及其还原作用最佳,而USWE法最差。

结论

USWE法提取挥发油的得率最高,且杂质较少,但还原能力和抑制作用最差。

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