Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, South Korea.
Exp Parasitol. 2011 Nov;129(3):260-5. doi: 10.1016/j.exppara.2011.07.017. Epub 2011 Jul 30.
Kelp grouper, Epinephelus bruneus, fed for 30 days with 0% (control), 0.1%, 1.0%, and 2.0% of Styrax japonica supplementation diets, led to reductions in mortality after being challenged with a bacterium (Vibrio harveyi) and a ciliate protozoan (Uronema marinum). The enriched diets significantly increased the survival rate as compared to the controls. The phagocytic and respiratory activities were significantly increased in kelp groupers given 1.0% and 2.0% enriched diets. The complement activity, lysozyme activity, serum bactericidal activity, and total protein level significantly increased with any enriched diet against the pathogens; however antiprotease activity and myeloperoxidase levels significantly increased only with 1.0% and 2.0% enriched diets while the α2-macroglobulin level was significantly enhanced with 1.0% enriched diet. The study suggests that incorporation of S. japonica at 1.0% and 2.0% level in the diet significantly enhances the immune responses in the kelp grouper E. bruneus against V. harveyi and U. marinum.
褐菖鲉，Epinephelus bruneus，连续 30 天投喂 0%（对照）、0.1%、1.0%和 2.0%的安息香树脂补充饲料，与细菌（哈维弧菌）和纤毛虫原生动物（海鱼波豆虫）攻毒后死亡率降低。与对照组相比，富含饲料的存活率显著增加。投喂 1.0%和 2.0%富含饲料的褐菖鲉的吞噬和呼吸活性显著增加。补充饲料对任何病原体的补体活性、溶菌酶活性、血清杀菌活性和总蛋白水平均显著增加；然而，仅 1.0%和 2.0%的富含饲料可显著增加抗蛋白酶活性和髓过氧化物酶水平，而 1.0%的富含饲料可显著提高α2-巨球蛋白水平。本研究表明，饲料中添加 1.0%和 2.0%水平的安息香树脂可显著增强褐菖鲉 E. bruneus 对哈维弧菌和海鱼波豆虫的免疫反应。
