Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, South Korea.
Fish Shellfish Immunol. 2011 Dec;31(6):801-7. doi: 10.1016/j.fsi.2011.07.017. Epub 2011 Jul 19.
Feeding kelp grouper, Epinephelus bruneus (26.1 ± 1.4), with 0%, 0.1%, 1.0%, and 2.0% Kalopanax pictus extract-supplementation diets, for 30 days reduced mortality on being challenged intraperitoneally 100 μl with bacterium Vibrio alginolyticus (2.1 × 10(7) cfu ml(-1)) and ciliate parasite Philasterides dicentrarchi (2.3 × 10(7) ciliates ml(-1)). The red blood cells (RBC), white blood cells (WBC), haemoglobin, haematocrit, lymphocytes, and monocytes levels significantly increased in kelp grouper fed with all doses of K. pictus-supplementation diets and challenged with bacterium and parasite when compared to control. However, the levels of mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), neutrophils, and thrombocytes did not significantly. The phagocytic activity, complement activity, and antiprotease activity did not significantly change in kelp grouper fed with 0.1% K. pictus-supplementation diets and challenged with bacterium and parasite. The respiratory activity, lysozyme activity, bactericidal activity, total protein level, and myeloperoxidase levels significantly increased in kelp grouper fed with all the doses of K. pictus-supplementation diet and challenged with bacterium and parasite. However, α2-macroglobulin level significantly increased with 1.0% diet, but not with 0.1% and 2.0% diets. Therefore this study suggests that 1.0% and 2.0% K. pictus-supplementation diets positively protected and enhanced the immune system in kelp grouper E. bruneus against V. alginolyticus and P. dicentrarchi infection.
投喂 26.1±1.4 体长的褐菖鲉（Epinephelus bruneus），30 天，分别用 0%、0.1%、1.0%和 2.0%高丽参提取物饲料，与用浓度为 2.1×10(7)cfu ml(-1)的弧菌（Vibrio alginolyticus）和浓度为 2.3×10(7)纤毛虫 ml(-1)的聚缩虫（Philasterides dicentrarchi）经腹腔内攻毒后，褐菖鲉的死亡率降低。与对照组相比，投喂高丽参提取物的褐菖鲉的红细胞（RBC）、白细胞（WBC）、血红蛋白、血细胞比容、淋巴细胞和单核细胞水平显著升高，且投喂高丽参提取物的褐菖鲉在经弧菌和寄生虫攻毒后，其吞噬活性、补体活性和抗蛋白酶活性均无显著变化。然而，投喂高丽参提取物对平均红细胞体积（MCV）、平均红细胞血红蛋白（MCH）、平均红细胞血红蛋白浓度（MCHC）、中性粒细胞和血小板水平无显著影响。投喂高丽参提取物的褐菖鲉，在经弧菌和寄生虫攻毒后，其呼吸活性、溶菌酶活性、杀菌活性、总蛋白水平和髓过氧化物酶水平显著升高。但是，1.0%高丽参提取物饲料使α2-巨球蛋白水平显著升高，而 0.1%和 2.0%高丽参提取物饲料则没有。因此，本研究表明，1.0%和 2.0%高丽参提取物饲料能对褐菖鲉（E. bruneus）的免疫系统产生积极的保护和增强作用，从而抵抗弧菌和聚缩虫感染。
