Paljärvi L, Naukkarinen A
Department of Pathology, University Central Hospital of Kuopio, Finland.
Histochemistry. 1990;93(4):385-7. doi: 10.1007/BF00315855.
Since capillary density of skeletal muscle depends on fiber type distribution, fiber typing should accompany measurement of capillary density. To obviate the need for multiple sectioning, we suggest NADH tetrazolium reductase enzyme histochemistry for fiber typing followed (on the same slide) by lectin histochemistry to demonstrate the binding of Ulex europaeus agglutinin, a sensitive marker for endothelium. The method is quick, highly reproducible and gives density estimates comparable to earlier, more tedious methods.
由于骨骼肌的毛细血管密度取决于纤维类型分布，因此纤维分型应与毛细血管密度测量同时进行。为避免多次切片的需要，我们建议采用NADH四氮唑还原酶酶组织化学进行纤维分型，随后（在同一张载玻片上）采用凝集素组织化学来显示欧洲荆豆凝集素的结合情况，欧洲荆豆凝集素是内皮细胞的一种敏感标记物。该方法快速、高度可重复，且密度估计结果与早期更繁琐的方法相当。
