Holthöfer H, Virtanen I, Kariniemi A L, Hormia M, Linder E, Miettinen A
Lab Invest. 1982 Jul;47(1):60-6.
Ulex europaeus I agglutinin, a lectin specific for some alpha-L-fucose-containing glycocompounds, was used in fluorescence microscopy to stain cryostat sections of human tissues. The endothelium of vessels of all sizes was stained ubiquitously in all tissues studied as judged by double staining with a known endothelial marker, antibodies against human clotting factor VIII. Cultured human umbilical vein endothelial cells, but not fibroblasts, also bound Ulex lectin. The staining was not affected by the blood group type of the tissue donor. In some tissues Ulex lectin presented additional binding to epithelial structures. Also, this was independent on the blood group or the ability of the tissue donor to secrete soluble blood group substances. Lotus tetragonolobus agglutinin, another lectin specific for some alpha-L-fucose-containing moieties failed to react with endothelial cells. Our results suggest that Ulex europaeus I agglutinin is a good histologic marker for endothelium in human tissues.
欧洲荆豆凝集素I是一种对某些含α-L-岩藻糖的糖化合物具有特异性的凝集素，在荧光显微镜检查中用于对人体组织的低温切片进行染色。通过与已知的内皮标记物——抗人凝血因子VIII抗体进行双重染色判断，在所有研究的组织中，各种大小血管的内皮均被普遍染色。培养的人脐静脉内皮细胞能结合荆豆凝集素，而成纤维细胞则不能。染色不受组织供体血型的影响。在一些组织中，荆豆凝集素与上皮结构有额外的结合。同样，这也与血型或组织供体分泌可溶性血型物质的能力无关。另一种对某些含α-L-岩藻糖部分具有特异性的凝集素——四角豆凝集素，未能与内皮细胞发生反应。我们的结果表明，欧洲荆豆凝集素I是人体组织内皮的良好组织学标记物。
