Department of Material & Life Chemistry, Faculty of Engineering, Kanagawa University, Yokohama 221-8686, Japan.
Chem Soc Rev. 2011 Dec;40(12):5855-66. doi: 10.1039/c1cs15149e. Epub 2011 Aug 8.
Pyrimidine base pairs in DNA duplexes selectively capture metal ions to form metal ion-mediated base pairs, which can be evaluated by thermal denaturation, isothermal titration calorimetry, and nuclear magnetic resonance spectroscopy. In this critical review, we discuss the metal ion binding of pyrimidine bases (thymine, cytosine, 4-thiothymine, 2-thiothymine, 5-fluorouracil) in DNA duplexes. Thymine-thymine (T-T) and cytosine-cytosine (C-C) base pairs selectively capture Hg(II) and Ag(I) ions, respectively, and the metallo-base pairs, T-Hg(II)-T and C-Ag(I)-C, are formed in DNA duplexes. The metal ion binding properties of the pyrimidine-pyrimidine pairs can be changed by small chemical modifications. The binding selectivity of a metal ion to a 5-fluorouracil-5-fluorouracil pair in a DNA duplex can be switched by changing the pH of the solution. Two silver ions bind to each thiopyrimidine-thiopyrimidine pair in the duplexes, and the duplexes are largely stabilized. Oligonucleotides containing these bases are commercially available and can readily be applied in many scientific fields (86 references).
嘧啶碱基对在 DNA 双链中选择性地捕获金属离子，形成金属离子介导的碱基对，可以通过热变性、等温滴定量热法和核磁共振波谱法进行评估。在这篇评论中，我们讨论了嘧啶碱基（胸腺嘧啶、胞嘧啶、4-硫代胸腺嘧啶、2-硫代胸腺嘧啶、5-氟尿嘧啶）在 DNA 双链中的金属离子结合。胸腺嘧啶-胸腺嘧啶（T-T）和胞嘧啶-胞嘧啶（C-C）碱基对分别选择性地捕获 Hg(II)和 Ag(I)离子，形成 DNA 双链中的金属基对 T-Hg(II)-T 和 C-Ag(I)-C。嘧啶-嘧啶碱基对的金属离子结合特性可以通过小的化学修饰来改变。通过改变溶液的 pH 值，可以改变 5-氟尿嘧啶-5-氟尿嘧啶对在 DNA 双链中的金属离子结合选择性。两个银离子结合到双链中的每个硫代嘧啶-硫代嘧啶对中，使双链得到很大的稳定。含有这些碱基的寡核苷酸是商业可获得的，并且可以容易地应用于许多科学领域（86 个参考文献）。
