DNA双链体中取代嘧啶碱基对的金属离子结合

Metal ion binding of substituted pyrimidine base pairs in DNA duplexes.

作者信息

Watanabe Yuko, Minowa Takahiro, Ono Akira

机构信息

Department of Chemistry, Graduate School and Faculty of Science, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachioji, Tokyo 192-0397, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2004(48):85-6. doi: 10.1093/nass/48.1.85.

DOI:10.1093/nass/48.1.85

PMID:17150490

Abstract

DNA duplexes containing 5-substituted uracil pairs and N4-substituted cytosine pairs have been chemically prepared and interactions between the duplexes and metal ions have been investigated. Substitutions at the 5-position with the electron withdrawing groups, such as bromo, fluoro and cyano groups, largely alter the binding selectivity of the duplexes and the metal ions.

摘要

含有5-取代尿嘧啶对和N4-取代胞嘧啶对的DNA双链体已通过化学方法制备，并研究了双链体与金属离子之间的相互作用。在5-位用吸电子基团如溴、氟和氰基进行取代，会极大地改变双链体与金属离子的结合选择性。