葡萄球菌肠毒素B的检测。酶联免疫吸附测定（ELISA）和酶标记免疫荧光分析（ELIFA）系统的比较研究。
Detection of staphylococcal enterotoxin B. A comparative study of ELISA and ELIFA systems.
作者信息
Dupont H, Therasse J, Pinon J M, Binder P
机构信息
Centre d'Etudes du Bouchet, Vert-le-Petit, France.
出版信息
J Immunol Methods. 1990 Apr 17;128(2):287-91. doi: 10.1016/0022-1759(90)90222-h.
The enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunofiltration assay (ELIFA) sandwich methods have been compared for their ability to detect staphylococcal enterotoxin B (SEB) in biological samples. Using the avidin-biotin amplification system in the ELISA, high sensitivity was observed and staphylococcal protein A interference was completely prevented. In contrast, ELIFA was less sensitive than ELISA, but very easy to perform, fast and simple to read routinely.
已对酶联免疫吸附测定（ELISA）和酶联免疫过滤测定（ELIFA）夹心方法检测生物样品中葡萄球菌肠毒素B（SEB）的能力进行了比较。在ELISA中使用抗生物素蛋白-生物素放大系统，观察到高灵敏度，并且完全防止了葡萄球菌蛋白A的干扰。相比之下，ELIFA的灵敏度低于ELISA，但操作非常简便，常规读取快速且简单。
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