Protein A insensitive ELISA detection of staphylococcal enterotoxin B.
作者信息
Rogemond V, Da Costa Castro J M, Delaunay T, Cazaux C, Guinet R M
机构信息
Centre d'Immunochimie Microbienne, Institut Pasteur de Lyon, L'Arbresle, France.
出版信息
Lett Appl Microbiol. 1991 Sep;13(3):175-8. doi: 10.1111/j.1472-765x.1991.tb00601.x.
Abstract
A sandwich enzyme-linked immunosorbent assay to detect staphylococcal enterotoxin B was developed using rat monoclonal antibodies as capture antibodies and as a biotinylated conjugate. This test was sensitive, less than 1 ng/ml of enterotoxin B was detected and interference by protein A was prevented by the use of rat monoclonal antibodies of the IgG2a isotype which were insensitive to protein A even at concentrations greater than 1000 ng/ml.
摘要
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