Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, Brazil.
Nat Prod Res. 2012;26(1):66-71. doi: 10.1080/14786419.2010.535160. Epub 2011 Aug 9.
The Lecythidaceae family is composed of 25 genera and 400 species that occur in the form of trees with a pantropical distribution. The genera Cariniana belongs to the family Lecythidaceae, and there are few reports considering these species. In this work, fractionation of the dichloromethane fraction obtained from the aqueous extract of the stem bark of Cariniana domestica (Mart) Miers, popularly known as Jequitibá-roxo, led to the isolation of two mixtures of triterpenoids: lupeol and β-amyrin and β-sitosterol and stigmasterol. The structures of the isolated compounds were elucidated by spectroscopic (NMR) and chromatographic (GC-MS) techniques as well as literature data comparisons. β-Sitosterol, stigmasterol, lupeol and β-amyrin were quantified in dichloromethane fraction by high-performance liquid chromatography (HPLC/DAD). The dichloromethane fraction was also investigated for antioxidant and antifungal activities. The isolated compounds and their biological activities are reported for the first time for the species C. domestica.
龙脑香科由 25 个属和 400 种组成，以热带分布的树木形式存在。栗属属于龙脑香科，对这些物种的研究报道较少。在这项工作中，从 Cariniana domestica（Mart）Miers 茎皮的水提物的二氯甲烷部分进行分段，得到两种三萜混合物：羽扇豆醇和 β-香树脂醇以及 β-谷甾醇和豆甾醇。通过光谱（NMR）和色谱（GC-MS）技术以及文献数据比较，阐明了分离化合物的结构。采用高效液相色谱法（HPLC/DAD）对二氯甲烷部分中的 β-谷甾醇、豆甾醇、羽扇豆醇和 β-香树脂醇进行定量分析。还研究了二氯甲烷部分的抗氧化和抗真菌活性。首次报道了分离得到的化合物及其对 C. domestica 物种的生物活性。
