Bioorganic Chemistry Division, Indian Institute of Integrative Medicine (CSIR), Jammu Tawi, India.
J Sep Sci. 2012 Feb;35(3):392-9. doi: 10.1002/jssc.201100647. Epub 2011 Dec 27.
A sensitive, selective and robust densitometric high-performance thin-layer chromatographic method was developed and validated for five marker compounds, namely betulin, lupeol, oleanolic acid, 3-acetyloleanolic acid and β-sitosterol, known for their various therapeutic activities. The marker compounds have been isolated from the stem bark of Betula utilis, well characterized by the spectral analysis, and their simultaneous quantitative determination carried out by high-performance thin-layer chromatography (HPTLC) method. The resolution of marker compounds was carried out on silica-gel 60 plates, using n-hexane:ethyl acetate (8:2 v/v) as the mobile phase. The HPTLC densitometry was performed at 500-nm wavelength after the post chromatographic derivatization with ceric ammonium sulfate reagent. The optimized method provided good linear relation (r>0.9960) for all the investigated analytes. The method is simple, and reproducible, which may be applied for quantitative analysis of the above-mentioned marker compounds.
建立并验证了一种用于五种标记化合物（白桦脂醇、羽扇豆醇、齐墩果酸、3-乙酰齐墩果酸和β-谷甾醇）的灵敏、选择性和稳健的密度测定高效薄层色谱法。这些标记化合物是从白桦的茎皮中分离出来的，通过光谱分析得到了很好的表征，并通过高效薄层色谱（HPTLC）法对其进行了同时定量测定。在硅胶 60 板上，使用正己烷：乙酸乙酯（8:2v/v）作为流动相进行标记化合物的分离。在与硫酸铈铵试剂进行柱后衍生化后，在 500nm 波长处进行 HPTLC 密度测定。对于所有研究的分析物，优化后的方法均提供了良好的线性关系（r>0.9960）。该方法简单、重现性好，可用于上述标记化合物的定量分析。
