Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute - FIOCRUZ, Brazil.
Virus Res. 2011 Sep;160(1-2):381-8. doi: 10.1016/j.virusres.2011.07.015. Epub 2011 Jul 30.
Genotype G5 group A rotavirus (RV-A), which is common in pigs and also detected in horses and cattle, circulated as endemic genotype in the 1980s and early 1990s in Brazil. After 1996, G5 RV-A has been replaced by G9 RV-A, becoming only sporadically detected. Recently, G5 has been reported in children with severe diarrhea in Argentina, Cameroon, Paraguay, People's Republic of China, and Vietnam, suggesting that, although uncommon in humans, it has a worldwide distribution. In a previous study, Brazilian G5 RV-A VP7 gene analysis demonstrated the existence of three main lineages: I, II, and III; all Brazilian strains and three porcine strains from Thailand grouped inside Lineage I. The VP8(*) subunit of VP4 gene showed that all P[8] strains fell into three major genetic lineages: P[8]-1; P[8]-2; and P[8]-3. Partial sequencing and phylogenetic analysis of VP1, VP2 and VP3 genes of P[8]G5 human RV-A strains were determined from 28 Brazilian strains collected from 1986 to 2005. The VP1-VP3 partial sequences analysis showed that the Brazilian strains have high amino acid identity with the human RV-A prototype IAL28 and other Wa-like genogroup strains. It was also shown that G5 RV-A Brazilian VP1-VP3 and VP7 sequences have a similar pattern of gouping: The study strains and the G5 prototype strain IAL-28 grouped together, while other prototypes, like OSU grouped separately. These results suggest that the core protein genes (VP1-VP3) of the G5 RV-A Brazilian strains might have originated from porcine and human strains. Phylogenetic analyses of VP7, VP4, VP1, VP2, and VP3 genes of P[8]G5 strains revealed a conserved genomic constellation (G5-P[8]-R1-C1-M1) with sequence similarity to Wa-like strains: IAL28, Wa, BE00048, CK00032, CK00033, DC4772 and DC1898, suggesting that despite the differences in genotypes (i.e., G5, G1 and G3) these viruses are genetically similar. The results presented here are fundamental to understand the epidemiology and evolution of genotype G5 RV-A and demonstrate the importance of continuous monitoring and molecular characterization of RV-A strains circulating in human and animal populations.
基因型 G5 组 A 轮状病毒（RV-A）在猪中很常见，也在马和牛中检测到，它作为地方性基因型在 20 世纪 80 年代和 90 年代初期在巴西流行。1996 年后，G5 RV-A 已被 G9 RV-A 取代，仅偶尔检测到。最近，阿根廷、喀麦隆、巴拉圭、中华人民共和国和越南的重症腹泻儿童中报告了 G5，表明尽管在人类中不常见，但它在全球范围内分布。在之前的一项研究中，对巴西 G5 RV-A VP7 基因分析表明存在三个主要谱系：I、II 和 III；所有巴西株和来自泰国的 3 株猪株都属于谱系 I。VP4 基因的 VP8(*)亚基表明，所有 P[8]株均分为三大遗传谱系：P[8]-1；P[8]-2；和 P[8]-3。对 1986 年至 2005 年期间从巴西收集的 28 株人 P[8]G5 RV-A 株的 VP1、VP2 和 VP3 基因进行了部分测序和系统进化分析。VP1-VP3 部分序列分析表明，巴西株与人类 RV-A 原型 IAL28 和其他 Wa 样基因群株具有高氨基酸同一性。还表明，G5 RV-A 巴西 VP1-VP3 和 VP7 序列具有相似的分组模式：研究株与 G5 原型株 IAL-28 聚集在一起，而其他原型株，如 OSU 则单独聚集。这些结果表明，G5 RV-A 巴西株的核心蛋白基因（VP1-VP3）可能源自猪和人株。对 P[8]G5 株的 VP7、VP4、VP1、VP2 和 VP3 基因进行的系统进化分析显示，具有与 Wa 样株（IAL28、Wa、BE00048、CK00032、CK00033、DC4772 和 DC1898）相似序列相似性的保守基因组结构（G5-P[8]-R1-C1-M1），表明尽管基因型（即 G5、G1 和 G3）不同，但这些病毒在遗传上相似。这里呈现的结果对于理解基因型 G5 RV-A 的流行病学和进化具有重要意义，并表明持续监测和对人类和动物人群中流行的 RV-A 株进行分子特征分析的重要性。
