Araújo Irene Trigueiros, Assis Rosane M Santos, Fialho Alexandre Madi, Mascarenhas Joana D'Arc P, Heinemann Marcos Bryan, Leite José Paulo G
Department of Virology, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
J Med Virol. 2007 Jul;79(7):995-1001. doi: 10.1002/jmv.20918.
Rotavirus epidemiological surveys with molecular analysis of strains are required for gastroenteritis control and prevention. Twenty-nine human rotavirus strains detected in Rio de Janeiro, Brazil, from 1986 to 2004 were characterized as P[8],G1, P[8],G5, P[8],G9, and P[4],G2 genotypes. The VP7 genes were sequenced and phylogenetic analysis was performed. Strains of genotype G1 revealed two distinct lineages, G1-3 and G1-4; strains of genotype G2 grouped in lineage G2-1; G5 strains clustered with other Brazilians G5 strains and G9 strains were closely related to each other in lineage G9-3, distinct from the original G9 strains detected in 1980s. The VP4 genes were analyzed and P[8] strains fell into two major genetic lineages, P[8]-2 and P[8]-3. Our findings document an intragenotype diversity represented by lineages and sublineages within rotavirus circulating in Rio de Janeiro from 1986 to 2004, before application of a vaccine (Rotarix) in Brazil. This report emphasizes the importance of continuing monitor genotypes to verify if uncommon strains or newly strains are emerging to be specifically addressed in future vaccine trials.
为了控制和预防肠胃炎，需要进行轮状病毒流行病学调查并对毒株进行分子分析。1986年至2004年在巴西里约热内卢检测到的29株人轮状病毒毒株被鉴定为P[8]、G1、P[8]、G5、P[8]、G9和P[4]、G2基因型。对VP7基因进行了测序并进行了系统发育分析。G1基因型毒株显示出两个不同的谱系，G1-3和G1-4；G2基因型毒株聚集在G2-1谱系中；G5毒株与其他巴西G5毒株聚类，G9毒株在G9-3谱系中彼此密切相关，与20世纪80年代检测到的原始G9毒株不同。对VP4基因进行了分析，P[8]毒株分为两个主要遗传谱系，P[8]-2和P[8]-3。我们的研究结果记录了1986年至2004年在巴西应用疫苗（Rotarix）之前里约热内卢流行的轮状病毒中由谱系和亚谱系代表的基因型内多样性。本报告强调了持续监测基因型以验证是否有罕见毒株或新毒株出现以便在未来疫苗试验中专门应对的重要性。
