Key Laboratory of Marine Bio-Resources Sustainable Utilization CAS, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1383-1388. doi: 10.1099/ijs.0.035071-0. Epub 2011 Aug 5.
A novel filamentous bacterium, strain SCSIO 10219(T), was isolated from a sediment sample collected from the South China Sea (113° 3.752' E 18° 1.722' N) at a depth of 2105 m. Growth was observed at 25-35 °C (optimum 30 °C) and pH 5.0-8.0 (optimum pH 6.0-7.0). The organism formed yellow-white colonies with radial wrinkles. Aerial mycelium was not produced on any of the growth media tested. Phenotypic characterization and 16S rRNA gene sequence analysis indicated that strain SCSIO 10219(T) belongs to the family Thermoactinomycetaceae. The strain contained ll-diaminopimelic acid in the cell wall. The predominant menaquinone was MK-7. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and five unknown phospholipids. Major fatty acids were anteiso-C(15:0) and iso-C(15:0). The DNA G+C content was 46.5 mol%. On the basis of chemotaxonomic properties and phylogenetic analysis based on 16S rRNA gene sequence data, it is proposed that this strain represents a novel species in a new genus, Marininema mesophilum gen. nov., sp. nov., in the family Thermoactinomycetaceae. The type strain of the type species is SCSIO 10219(T) ( = CCTCC AA 2011006(T) = DSM 45610(T)). In addition, we propose that the description of the family Thermoactinomycetaceae should be further emended based on the present study.
一株新的丝状细菌，菌株 SCSIO 10219(T)，是从南海（东经 113°3.752'，北纬 18°1.722'）水深 2105 米的沉积物样本中分离出来的。在 25-35°C（最佳 30°C）和 pH5.0-8.0（最佳 pH6.0-7.0）的条件下可以观察到生长。该菌形成具有放射状皱纹的黄白色菌落。在所有测试的生长培养基中均未产生气生菌丝。表型特征和 16S rRNA 基因序列分析表明，菌株 SCSIO 10219(T) 属于高温放线菌科。该菌株的细胞壁含有 ll-二氨基庚二酸。主要的类异戊二烯醌为 MK-7。磷脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰乙醇胺和五种未知磷脂。主要脂肪酸为 anteiso-C(15:0)和 iso-C(15:0)。DNA G+C 含量为 46.5mol%。基于化学分类特性和基于 16S rRNA 基因序列数据的系统发育分析，建议该菌株代表一个新属中的一个新种，嗜中温 Marininema mesophilum 属，种名。模式种的模式菌株是 SCSIO 10219(T)（= CCTCC AA 2011006(T)=DSM 45610(T)）。此外，我们建议根据本研究进一步修订高温放线菌科的描述。
