Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4562-4567. doi: 10.1099/ijs.0.052142-0. Epub 2013 Aug 1.
A novel Gram-stain-positive bacterium, designated strain YIM M11385(T), was isolated from a marine sediment sample collected from the South Bay, Little Andaman Island, India with a salinity of 35 p.p.m., pH 8.5. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM M11385(T) belongs to the genus Marininema, supported by a bootstrap value of 100 %. The taxonomic position of this organism was further established by using a polyphasic approach. Strain YIM M11385(T) grew optimally at 28 °C, pH 7.0 and in the presence of 0-5 % (w/v) NaCl. The 16S rRNA gene sequence similarity between strain YIM M11385(T) and Marininema mesophilum SCSIO 10219(T) was 98.3 %. Strain YIM M11385(T) exhibited a quinone system with only MK-7, the polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as major components, and the major fatty acids were anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The level of DNA-DNA relatedness between strain YIM M11385(T) and M. mesophilum SCSIO 10219(T) was 59.36 %. On the basis of genotypic and phenotypic data, it is apparent that strain YIM M11385(T) represents a novel species of the genus Marininema, for which the name Marininema halotolerans sp. nov. is proposed. The type strain is YIM M11385(T) ( = CCTCC AB 2012052(T) = DSM 45789(T)). In addition, we propose that the description of the genus Marininema should be further emended based on the results of the present study.
一株新型革兰氏阳性细菌，菌株 YIM M11385(T)，从印度小安达曼岛南湾的海洋沉积物样本中分离得到，该样本的盐度为 35 p.p.m.，pH 值为 8.5。基于 16S rRNA 基因序列的系统发育分析表明，菌株 YIM M11385(T)属于 Marinimema 属，支持该分类地位的bootstrap 值为 100%。通过多相分类法进一步确定了该生物的分类地位。菌株 YIM M11385(T)在 28°C、pH 值 7.0 和 0-5%（w/v）NaCl 存在的条件下生长最佳。菌株 YIM M11385(T)与 Marinimema mesophilum SCSIO 10219(T)的 16S rRNA 基因序列相似度为 98.3%。菌株 YIM M11385(T)的醌类系统只有 MK-7，极性脂类谱包括二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺作为主要成分，主要脂肪酸为 anteiso-C15:0、iso-C15:0、anteiso-C17:0 和 iso-C16:0。菌株 YIM M11385(T)与 Marinimema mesophilum SCSIO 10219(T)的 DNA-DNA 同源性水平为 59.36%。基于基因型和表型数据，菌株 YIM M11385(T)明显代表 Marinimema 属的一个新种，因此建议将其命名为 Marinimema halotolerans sp. nov.。模式菌株为 YIM M11385(T)（ = CCTCC AB 2012052(T) = DSM 45789(T)）。此外，我们建议根据本研究的结果进一步修订 Marinimema 属的描述。
