Kosiewicz M M, Michael S D
Department of Biological Sciences, State University of New York, Binghamton 13901.
J Reprod Fertil. 1990 Mar;88(2):427-40. doi: 10.1530/jrf.0.0880427.
Using a variation on a standard follicle classification technique, 5 classes of follicles were quantified in serial sections of ovaries from intact mice and mice thymectomized on Day 3 at 5-day intervals from 5 to 40 days of age. Sera from these animals and from animals 60 days of age were analysed for the presence of anti-oocyte antibodies. Ovaries from intact animals 10 to 40 days of age were examined for the presence of antigen(s) using anti-oocyte antibody-positive sera from all ages of mice. There was a dramatic decrease in the primordial follicle population at 10 days of age in thymectomized mice and that population remained significantly lower until 40 days of age. The growing follicle population was also significantly lower at 20 days of age in thymectomized mice and remained lower through 40 days of age. Anti-oocyte antibodies were not detectable until 30 days of age and at that age reacted with oocytes from all follicle types including primordial. Ovarian antigens were present in similar patterns in ovaries from mice at all ages tested. We conclude that thymectomy has an earlier influence on the ovary than previously thought and this influence does not appear to involve the immune activity associated with autoimmune ovarian dysgenesis. This suggests that the effect of thymectomy on the ovary may be biphasic: (1) an early effect, possibly involving a disruption in the hypothalamic-pituitary-ovarian-thymic axis, that influences the primordial and growing follicle populations before 20 days of age; and (2) a later effect involving an immune imbalance first evident by 25-30 days of age that ultimately results in the destruction of the ovary.
运用一种标准卵泡分类技术的变体，在5至40日龄的完整小鼠以及3日龄时进行胸腺切除且每隔5天取材直至40日龄的小鼠卵巢连续切片中，对5类卵泡进行了定量分析。分析了这些动物以及60日龄动物血清中抗卵母细胞抗体的存在情况。使用各年龄段小鼠抗卵母细胞抗体阳性血清，检测10至40日龄完整动物卵巢中抗原的存在情况。胸腺切除小鼠在10日龄时原始卵泡数量急剧减少，且该数量在40日龄前一直显著低于正常水平。胸腺切除小鼠在20日龄时生长卵泡数量也显著减少，并在40日龄时一直维持在较低水平。抗卵母细胞抗体直到30日龄才被检测到，且在该年龄与包括原始卵泡在内的所有卵泡类型的卵母细胞发生反应。在所有测试年龄的小鼠卵巢中，卵巢抗原呈现相似的模式。我们得出结论，胸腺切除对卵巢的影响比之前认为的更早，且这种影响似乎并不涉及与自身免疫性卵巢发育不全相关的免疫活性。这表明胸腺切除对卵巢的影响可能是双相的：（1）早期影响，可能涉及下丘脑 - 垂体 - 卵巢 - 胸腺轴的破坏，在20日龄前影响原始卵泡和生长卵泡的数量；（2）后期影响，涉及免疫失衡，在25 - 30日龄时首次明显，最终导致卵巢破坏。
